ASSESSMENT OF MILK METABOLOMICS AND PHYSIOBIOCHEMICAL PROFILES IN ZINC SUPPLEMENTED DAIRY GOATS DURING WINTER AND SUMMER SEASONS
| dc.contributor.advisor | ANJALI AGGARWAL | |
| dc.contributor.author | POOJA ANAND | |
| dc.date.accessioned | 2023-12-01T14:26:26Z | |
| dc.date.available | 2023-12-01T14:26:26Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Present study was carried out on crossbred lactating goat (Capra hircus) to study the effect of zinc supplementation on milk metabolites and physio-biochemical changes during winter and summer seasons. Eighteen crossbred Alpine × Beetal (AB) goats were selected and divided into three groups, 6 animals each as Control (C: no zinc supplementation), Treatment 1 (T1: 30ppm of zinc supplementation) and Treatment 2 (T2: 50ppm of zinc supplementation) on DMI basis during winter and summer seasons. Milk and blood samples were collected at fortnight interval. The physiological, hematological, bio-chemical and hormonal parameters as well as milk yield, milk composition and milk minerals content in goat were investigated during both the seasons. The present study intended to investigate the changes in milk metabolites if any in zinc supplemented dairy goats. One-way ANOVA analysis revealed no significant difference between the metabolites in milk samples of control, T1 and T2 groups. However, PLS-DA analysis revealed twenty-five (25) metabolites found in control, T1 and T2 groups of milk samples were having VIP score ≥ 1. Based on -log10(p) and impact values, pathways such as alanine, aspartate and glutamate metabolism, biosynthesis of unsaturated fatty acid, TCA cycle, linoleic acid metabolism, glyoxylate and dicarboxylate metabolism, glutathione metabolism, and glycine, serine and threonine metabolism were found to be differentially regulated in control, T1 and T2 groups and these were involved in carbohydrate, lipid, amino acid and nucleotide metabolism. No significant effect of zinc supplementation was observed on rectal temperature, respiration rate, pulse rate and skin temperature in control, T1 and T2 groups but significantly (p<0.05) higher values were observed during summer as compared to winter. No significant effect of zinc supplementation was observed on hematological parameters (Hb, PVC and TEC), milk yield and milk composition (fat, SNF, protein and lactose) but their values were significantly (p<0.05) higher in control, T1 and T2 groups during winter as compared to summer. No significant effect of zinc supplementation and seasonal variation was observed on milk mineral content of sodium, potassium and zinc but significantly (p<0.05) higher values of milk calcium were observed in control, T1 and T2 groups during winter as compared to summer. Plasma cortisol was found significantly (p<0.05) lower in T2 group during summer season, whereas plasma thyroxine was found significantly (p<0.05) higher in T2 group during winter season. A significantly (p<0.05) higher level of plasma insulin was observed in control and treatment (T1 and T2) groups of AB goats during summer season, whereas no effect of zinc supplementation and seasonal variation was observed on plasma prolactin levels. No significant change in plasma glucose level was observed in control and supplemented groups (T1 and T2) but during winter season, significantly (p<0.05) higher plasma glucose levels (mg/dl) was observed. A significant (p<0.05) increase in plasma zinc levels in T2 group, during the IV and V fortnight intervals of experimental period during summer season was observed. A significantly (p<0.05) higher values of plasma copper level (ppm) in control group as compared to T2 group, whereas no significant variation between the supplemented groups (T1 and T2) was found during summer season. Based on present study, it can be concluded that supplementation of 50ppm of zinc was better in ameliorating effect of heat stress in goats. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810202011 | |
| dc.language.iso | English | |
| dc.pages | 149 p. | |
| dc.publisher | ICAR-NDRI, KARNAL | |
| dc.sub | Animal Physiology | |
| dc.theme | ANIMAL PHYSIOLOGY | |
| dc.these.type | M.V.Sc. | |
| dc.title | ASSESSMENT OF MILK METABOLOMICS AND PHYSIOBIOCHEMICAL PROFILES IN ZINC SUPPLEMENTED DAIRY GOATS DURING WINTER AND SUMMER SEASONS | |
| dc.type | Thesis |