STUDIES ON IN VITRO PROPAGATION OF CARNATION (Dianthus caryophyllus L.)
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Date
2015
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ABSTRACT The present investigation entitled, “Studies on in vitro propagation of Carnation (Dianthus caryophyllus L.)” was carried out at the Plant Tissue Culture Laboratory and Experimental Farm of Department of Floriculture and Landscape Architecture, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan (HP) during 2014-2015. The experiments were laid out in a Completely Randomized Design (factorial) consisting of two cultivars i.e. ‘Parendillo’ and ‘Yellow Star’. Out of the two carnation cultivars under study, ‘Parendillo’ showed superiority over ‘Yellow Star’ for all the characters observed during in vitro propagation. Shoot tips were rated better explants than nodal sections for mass multiplication in carnation cultivars. A protocol was developed for production of high quality virus indexed carnation plants in vitro. As a refinement in already existing protocol, 5 % Calcium Hypochlorite treatment of explants is suggested as a potential substitute for Mercuric Chloride, a hazardous chemical used previously for surface sterilization. 100 % uncontaminated growing cultures in cv.’Parendillo’ and. ‘Yellow Star’ were obtained with 5 % Calcium Hypochlorite treatment of explants for 10 and 15 minutes, respectively. For culture establishment, MS medium supplemented with 2.5 mgl-1 BA showed maximum per cent response. High quality shoots could be produced from shoot tip raised shoots in multiplication medium containing 2 mgl-1 BA, 0.1 mgl-1 NAA and 1 mgl-1 GA3. The in vitro multiplied shoots were serologically indexed using DAS- ELISA for important carnation viruses viz. Carnation Latent Virus (CLV), Chrysanthemum Virus B (CVB) and Chrysanthemum Aspermy Virus (CAV). All the shoots showing multiplication under in vitro conditions were tested free of viruses and put to rooting. MS medium containing 2 mgl-1 NAA and 0.1 % activated charcoal was found to be the best for in vitro rooting of multiplied shoots. The in vitro rooted plantlets showed 100% survival and maximum growth of plantlets hardened in a mixture of cocopeat : sand : perlite (2:2:1, v/v).