SURVEY, BIOLOGICAL & MOLECULAR CHARACTERIZATION AND MANAGEMENT OF CUCUMBER MOSAIC VIRUS IN GHERKIN (Cucumis anguria L.)
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Date
41834
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Publisher
University of Agricultural Sciences GKVK, Bangalore
Abstract
Cucumber mosaic virus (CMV) is most destructive disease on gherkin. Survey
during 2013-14 revealed that, disease incidence ranging from 16.00 to 59.65 per cent
with the highest incidence recorded in Kolar (51.27%) and least in Bangalore rural
(17.15%). The virus was readily transmitted by sap and aphids on gherkin cv. green long
and showed systemic mosaic with dark and light green or with yellow patches with slight
puckering. A single vector, Aphis gossypii per plant was sufficient to transmit disease
upto 20 per cent, whereas, 100 per cent transmission was obtained in A. gossypii and 90
per cent in Myzus persicae when 5 and 10 aphids per plant were used with 10 min.
acquisition and inoculation access period. Ash gourd, Bottle gourd, Bush squash,
Cucumber, Muskmelon, Pumpkin, Ridge gourd, Round melon and Chilli showed mosaic
symptoms, Tomato showed shoestring symptom, Chenopodium and Cowpea showed
necrotic local lesions upon inoculation with CMV. Virus reacted strongly to cucumovirus
specific antisera through DAS-ELISA. PCR product of CMV infected sample with CPprimers
indicated ~700bp-DNA and showed high nucleotide homology with the strains of
CMV subgroup-IB (95-99%) and sequence conservation of nucleotide (99%) with ANstrain
(subgroup-IB). CMV in gherkin was effectively managed by border crop African
tall maize + reflective mulch + spraying Lastraw (0.5%) + Neemark (0.5%) + Groundnut
oil (1%) at 15 days intervals which recorded the lowest mean per cent incidence (8.05)
compare to control (36.00). Out of 43 genotypes screened, Hyb.11 genotype showed
immune reaction to CMV.