STUDIES ON in vitro PROPAGATION AND CONSERVATION OF Viola pilosa Blume
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Date
2010
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UHF,NAUNI,SOLAN
Abstract
Abstract
A protocol for in vitro propagation and conservation was developed for Viola pilosa, a
valuable medicinal plant The sterilized explants (buds) cultured on MS medium supplemented with 1
mg/l BA, 1 mg/l TDZ and 0.50 mg/l GA3 gave best results for in vitro shoot bud establishment.
Although the problem of shoot vitrification occurred on this medium but this was overcome by
transferring the vitrified shoot on MS medium supplemented with 1 mg/l BA and 0.25 mg/l Kn. The
same medium was found out to be the best medium for in vitro shoot multiplication. Also effect of
different types of solidifying agents on the growth of in vitro multiplying shoots was studied and out of
three different solidifying agents viz. agar agar, agarose and gelrite gellan gum, the gelrite gellan gum
containing medium was found out to be the best medium for in vitro shoot multiplication. Callus was
obtained as a by product from shoots on MS medium supplemented with 1 mg/l BA, 1 mg/l TDZ and
0.50 mg/l GA3 and this callus was further multiplied on medium supplemented with 1.5 mg/l NAA.
The shoot regeneration was obtained from the callus on MS basal medium supplemented with 0.1%
activated charcoal. 100% root induction from in vitro grown shoots was obtained on half strength MS
medium supplemented with 1 mg/l IBA.
In vitro conservation was successfully demonstrated by using two different approaches
namely slow growth at low temperature and cryopreservation following vitrification. In slow growth at
low temperature studies the in vitro shoots incubated at 10oC, though showed 100% retrieval,
however, shoots became etiolated, while the shoots incubated at 4oC showed retrieval of 85.7% with
no morphological variation during incubation. In cryopreservation - vitrification studies the vitrified
shoot buds gave maximum retrieval of 41.66% when they were precooled at 4oC while only 16.66%
vitrified shoots were retrieved from those precooled at 10oC. In vitro formed plantlets were hardened
and transferred to soil with 55.55% survival.
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