Targeting genes for ion homeostasis and salt tolerance in finger millet (Eluesine coracana (L) Gaertn. ): Over-expressing genes for salt compartmentation and proton gradient generation and their significance in salt tolerance
| dc.contributor.advisor | V. R, Shashidhar | |
| dc.contributor.author | K. M, Vasantha | |
| dc.date.accessioned | 2016-05-24T09:17:03Z | |
| dc.date.available | 2016-05-24T09:17:03Z | |
| dc.date.issued | 2013-12-12 | |
| dc.description.abstract | Salinity in agricultural terms is the excess of salts above the level plant requirement. Most often it poses constraints in the growth hence productivity of the crop plants gets affected and therefore is a serious concern. Salinity is one of the major abiotic stresses affecting plant productivity. Finger millet (Eluesine coracana (L.)Gaertn), is grown on over 4 million hectare worldwide and it is a primary food for millions in dry lands and is sensitive to moderate levels of salt in the soil. To generate finger millet plants that can adapt to saline soil, AVP1, a vacuolar H+-pyrophosphatase gene from Arabidopsis thaliana, and PgNHX1, a vacuolar Na+/H+antiporter gene from Pennisetum glaucum, were co-expressed by Agrobacterium tumefaciens-mediated transformation and attempts has been made on standardization of an efficient direct regeneration protocol in finger millet. The shoot meristem was found to be the best responsive explants for direct organogenesis. The functional significance of AVP1, NHX1 and AVP1::NHX1 transgenic plants was demonstrated invitro and invivo by using Hydroponics’ systems, gravimetric analysis and soil system by Karnal method. Physiological studies such as CMS, Nutrient estimation of Na, K, and Ca, chlorophyll stability index, Standard Evaluation Score for visual salt injury, Proline, OA and yield analysis. It is reported here that co-expression of AVP1 and PgNHX1 confers enhanced salt tolerance to the transformed finger millet compared with the AVP1 and PgNHX1 single gene transgenic plants and the wild-type. These transgenic plants grew well in the presence of 20 dS/m NaCl while wild-type plants exhibited chlorosis and died within the 30days.The physiological analysis of the transgenic lines clearly demonstrates that co-expression of AVP1 and PgNHX1 improved the osmoregulatory capacity of double transgenic lines by enhanced sequestration of ions into the vacuole from cytosolby increasing the availability of protons and thus alleviating the toxic effect of Na+ | en_US |
| dc.identifier.other | Th-10709 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/66240 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Agricultural Sciences, Bengaluru | en_US |
| dc.sub | Crop Physiology | |
| dc.subject | planting, millets, transgenics, genes, tolerance, food preservation, genetic processes, vegetative propagation, regeneration, thinning | en_US |
| dc.these.type | Ph.D | |
| dc.title | Targeting genes for ion homeostasis and salt tolerance in finger millet (Eluesine coracana (L) Gaertn. ): Over-expressing genes for salt compartmentation and proton gradient generation and their significance in salt tolerance | en_US |
| dc.type | Thesis | en_US |