MOLECULAR EVALUATION OF MUNGBEAN (Vigna radiata (L.) Wilczek) RIL POPULATION DERIVED FROM CROSS BETWEEN CHINAMUNG AND BL849 FOR POWDERY MILDEW DISEASE RESISTANCE
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Date
2019-08-27
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
Mungbean (Vigna radiata (L). Wilczek) is a kharif pulse crop cultivated for its
seeds and also as green manuring crop for soil enrichment. The yield in case of
mungbean is greatly affected by numerous abiotic and biotic factors. Among biotic
factors, Powdery mildew is one of the foliar disease caused by the fungal pathogen
Erysiphe polygoni DC, that incidence results in the 20-40 per cent of yield reduction. The
parental lines and two checks (Chinamung, BL849 and KKM-3, Pusa baisaki) and 96 F8
Recombinant Inbrid Lines (RILs) were sown in field for powdery mildew disease
screening based on five per cent scale to identify highly resistant and highly susceptible
RILs. Among 96 RILs, nine are moderately resistant, sixty are moderately susceptible,
twenty-four are susceptible and three are highly susceptible. All the 96 RILs were
phenotyped for 12 quantitative traits under field conditions. The GCV and PCV were
found higher for important yield contributing traits and seed yield is positively correlated
with major yield contributing traits. The parental polymorphism study showed only 14
are found polymorphic out of 248 primers. Among the 14 polymorphic primers only one
primer DMBSSR130 showed segregation in Bulk Segregant Analysis (BSA). Single
Marker Analysis (SMA) revealed that two primers (DMBSSR130 and DMBSSR125) was
significantly linked to powdery mildew resistant gene with R2
value of 79 per cent and 73
per cent respectively