In vitro propagation and phyotochemical profiling of Rauwolfia serpentina (Sarpagandha)
| dc.contributor.advisor | Dhiman, Karuna | |
| dc.contributor.author | Sharma, Shipra | |
| dc.date.accessioned | 2023-07-17T09:25:04Z | |
| dc.date.available | 2023-07-17T09:25:04Z | |
| dc.date.issued | 2022-04-13 | |
| dc.description.abstract | In the present investigation a technique for in vitro propagation and phytochemical profiling ofRauwolfia serpentina (Sarpagandha) has been developed. Axillary buds, shoot tips and leaves from maintained mother plants of Sarpgandha were used as the source of explants. Treatment of different explants with 1 % HgCl2 for 30 seconds proved to be best as it gave maximum 80 % uncontaminated explants with 65 % culture survival rate. Maximum in vitro axillary bud proliferation (76%) on MS medium supplemented with 1.0 mg/l Zeatin and 2.0 mg/l IBA whereas MS medium fortified with BAP (2.0 mg/l) + Kn (2.0 mg/l) proved to be best for shoot tips regeneration with 87.55% maximum regeneration frequency. Maximum indirect shoot regeneration (89 %) was observed using leaf as explants on MS medium containing 2.0 mg/l BAP and 1.0 mg/l NAA. Shoots were multiplied further on MS medium containing2.5 mg/l NAA + 1.0mg/l BAP. Microshoots were cultured on half strength MS media for in vitro rooting and 82% root induction was achieved when IBA (1.5mg/l) and IAA (1.5mg/l) was used in combination. Well developedin vitro raised plantlets were hardened in potting mixture containing soil: sand: vermiculite in 1:1:1 ratio. Phytochemical profiling of in vitro raised plantlets as well as mother plants was carried out qualitatively, quantitatively and by TLC analysis. Alkaloid , flavonoids, tannin and phenolic compounds, phytosterols and saponins were present in leaves and roots of in vitro raised plant. TLC analysis also showed presence of reserpine in root samples ofin vitroraised plants. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810198305 | |
| dc.keywords | vitro propagation and phyotochemical, Rauwolfia serpentina (Sarpagandha),Genetics, Biotechnology | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 51+vii | en_US |
| dc.publisher | College of Horticulture and Forestry, Dr YSP UHF, Neri, Hamirpur (H.P.) | en_US |
| dc.sub | Biotechnology | en_US |
| dc.theme | Propagation and phytochemical profiling of Rauwolfia serpentina | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | In vitro propagation and phyotochemical profiling of Rauwolfia serpentina (Sarpagandha) | en_US |
| dc.type | Thesis | en_US |
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