CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH MAIZE (Zea maysL.) UNDER LOW HILL CONDITIONS OF HIMACHAL PRADESH
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Date
2014
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UHF,NAUNI
Abstract
ABSTRACT
Plant growth promoting rhizobacteria (PGPR) are used as inoculants for improving
the growth and yield of agricultural crops. PGPR are also low cost eco-friendly inoculants for
detoxification and bioremediation of soil. The present investigation was carried out during
the Kharif seasons of 2013-14 with the objectives to develop a potential bio-inoculum/ bio
control agent for maize crop growing in low hill conditions of Himachal Pradesh. The
rhizospheric and root samples of maize (Zea mays L.) plants were collected from Hamirpur
(31.63˚ N to 76.51˚ E), Bilaspur (31.33˚ N to 76.75˚ E) and Una (31.47˚ N to 76.27˚ E)
districts of Himachal Pradesh to isolate an effective inoculums of PGPR with multiple plant
growth promoting traits (P-solubilization, N-fixation, IAA, Siderophore, HCN production
and antagonism against soil borne pathogens). Five isolates (B1N1, J2, J4, 3M and R6)
possesses maximum plant growth promoting traits were selected. On the basis of its
morphological, physiological and biochemical characteristics these five isolates were
tentatively identified as Bacillus sp. Seed bacterized with isolate B1N1 showed significant
increase in germination, leaf number, shoot length,shoot biomass, root length, root biomass
over uninoculated control. The seed inoculated withB1N1 registered significant increase in
NPK uptake by 78.22, 74.63, 80.77 per cent, respectively over uninoculated control. These
two (B1N1 and J2) potential isolates was also identified by 16S rRNA gene sequencing as
Bacillus subtilis. Biofertization and bioprotactant potential of selected bacterial isolates paved
the way of their use at commercial level to supplement chemical inputs (Fertilizer and
fungicide) for enhance production.
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