IDENTIFICATION OF DNA MARKERS ASSOCIATED WITH GRAIN FE, ZN AND PROTEIN CONTENTS IN RICE (oryza sativa L.)
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Date
2017
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Indira Gandhi Krishi Vishwavidhyalaya, Raipur
Abstract
Rice is the primary or secondary stable food for 50 % of the world population. Rice contributes major on source of calories for rice eating population. Over 800 million people in developing world are undernourished suffering from either protein energy or micro-nutrient deficiency. There is a serious need to redesign the way that will ensure the balance nutrient supply of major stable food for people in adequate and affordable amount. To improve nutritive value of rice the preliminary step is to characterized genetic variability for iron, zinc and grain protein content in germplasm and then to use this variability for breeding nutrient rich. Looking to this present study entitled “Identification of DNA Markers Associated with Grain Fe, Zn and Protein Contents in Rice (Oryza sativa L.)” was conducted at the department of Plant Molecular Biology and Biotechnology, Indra Gandhi Krishi Vishwavidyalaya, Raipur. markers have proven to be powerful tools in the assessment of genetic variation and in the elucidation of genetic relationships within and among species ( Shivapriya and Hittalmani, 2006).Hence the present study was undertaken in order to identify the molecular markers associated with micronutrient and grain protein of rice along with Initially 96 rice were characterized for 17 morphological trait, phenotyping for major 8 quantitative and 9 qualitative traits. A set of 96 rice genotypes including landraces, breeding lines and cultivars were screened for the micronutrient and protein contents wide genetic variables for all trait i.e. Fe, Zn and protein were recorded among the tested rice genotypes, which ranged from14.3 ppm (Bisni) to 4.7 ppm (IR681444*Moro) Fe, 34.8 ppm (Dullar) to13.6 ppm (GP-145-138) for Zinc and 10.28% (Kalam Gurmatia) to 5.78%(CGR-1539) for grain protein content. The rice genotypes processing higher grain Fe, Zn and protein contents were identified as donor rice genotypes to be useful in breeding.
Molecular markers, a powerful tool for assessing the genetic variability were also used to characterization the rice genotypes out of total 42 SSR marker used in this study 18 were found to be polymorphic in nature, and only 8 were found to be associated with grain nutritive value i.e. Fe, Zn and protein .In which 8 marker RM5, RM19, RM154, RM234, RM279, RM490, RM225, and Crm33-1are to be associated with Iron, Zinc and grain protein content of rice present on chromosome number 1,12,2,7,2,1,6 and 3.the associated markers identified for grain Fe, Zn and protein content can be further useful in marker assisted selection and speedy development of nutri-rich rice varieties to compact malnutrition.
Description
IDENTIFICATION OF DNA MARKERS ASSOCIATED WITH GRAIN FE, ZN AND PROTEIN CONTENTS IN RICE (oryza sativa L.)
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