Development of transgenic pigeon pea (Cajanus cajan L.) plants containing Lectine receptor like kinase gene for improving abiotic stress tolerance

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dc.contributor.advisorKharb, Pushpa
dc.contributor.authorPratibha
dc.date.accessioned2020-11-11T07:13:33Z
dc.date.available2020-11-11T07:13:33Z
dc.date.issued2019-05
dc.description.abstractPigeon pea (Cajnus cajan L.) is a nutrient rich, sixth most important and second most important legume pulse crop of the world and India respectively after chickpea. Production and productivity of pigeon pea is affected by various abiotic stresses but salinity is the most deleterious stress which causes considerable loss in crop’s productivity. In the present study, transgenic pigeon pea plants (cv.. Manak) carrying OsLecRLK gene, were developed for salinity tolerance using Agrobacterium tumefaciens strain LBA4404 with pCAMBIA1300- OsLecRLK gene (Kharb et al., 2018 Patent Application No. 201811012099). Putative transformants were screened through PCR amplification using gene specific primers and out of 86 plants 16 plants were found positive giving a transformation efficiency of 18.6%. Six higher yielding lines were selected and checked for stable transgene integration. Southern hybridization and Real-time PCR analysis was done to find out the copy no. of transgene in selected transgenic lines. Seeds from T0 pigeon pea plants were sown to raise T1 generation and a total of 52 plants (out of 120) showed the presence of OsLecRLK gene. Physiobiochemical analysis was done to assess the efficacy of transgene via subjecting wild type and selected T1 transgenic plants to 75 mM salt stress. The results showed that transgenic line performed better in terms of maintaining higher chlorophyll content, relative water content, proline content, total soluble sugar content, peroxidase and catalase activity in comparison to the wild type plants. Moreover, MDA content and membrane injury index were significantly reduced in transgenic lines when compared to the wild type plants indicating that the transgenic lines were less affected by salt stress. Among all transgenic lines, L-19 performed well with respect to all the parameters studied and can be taken further for the development of transgenic pigeon pea plants for salt stress tolerance.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810154904
dc.keywordsPigeon pea, transgenic, OsLecRLK gene, Agrobacterium, salinity stress, physio-biochemicalen_US
dc.language.isoEnglishen_US
dc.pages49+ xen_US
dc.publisherCCSHAU, Hisaren_US
dc.subBiotechnology and molecular Biologyen_US
dc.themeDevelopment of transgenic pigeon pea (Cajanus cajan L.) plants containing Lectine receptor like kinase gene for improving abiotic stress toleranceen_US
dc.these.typePh.Den_US
dc.titleDevelopment of transgenic pigeon pea (Cajanus cajan L.) plants containing Lectine receptor like kinase gene for improving abiotic stress toleranceen_US
dc.typeThesisen_US
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