Generation of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbean

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dc.contributor.advisorBhat, Sumangala
dc.contributor.authorPooja S.K.
dc.date.accessioned2022-01-27T04:41:04Z
dc.date.available2022-01-27T04:41:04Z
dc.date.issued2017-07
dc.description.abstractThe present study was conducted to evaluate mungbean accessions for powdery mildew resistance, identify polymorphic markers between powdery mildew resistant and susceptible parents and validate molecular markers previously reported to be associated with powdery mildew resistance, at the University of Agriculture Sciences, Dharwad. Phenotypic screening of 130 mungbean accessions (procured from IIPR, Kanpur and AICRP on MULLaRP, UAS, Dharwad) including susceptible check (DGGV2) under natural epiphytotic condition was carried out at the Main Agriculture Research Station during kharif and rabi, 2016 using augmented design. During kharif, four genotypes (NUL- 7, GPM- 19, V. umbellata and V. trilobata) showed immune response, three genotypes viz., COGG- 913, VAIBHAV and TARM1 showed moderate resistance, six genotypes were rated as susceptible and the remaining 119 genotypes were rated as highly susceptible. During rabi, only three genotypes viz., GPM-19, V. umbellata and V. trilobata showed immune response, four genotypes (NUL-7, COGG-913, TARM1 and Vaibhav) were moderately resistant, nine genotypes showed susceptible reaction and the remaining 114 genotypes were rated as highly susceptible to the disease. High heritability and genetic advance over mean was observed for percent disease index (PDI) among 130 mungbean accessions. The molecular experiments were conducted in the Department of Biotechnology. Out of 64 SSR markers used for polymorphism survey between powdery mildew susceptible (DGGV2) and previously identified resistant (TARM1) parents, only four markers (CEDG121, CEDG245, MB-SSR238 and GMES5773) showed polymorphism. F1s were confirmed using polymorphic marker (MB-SSR238) and confirmed F1s were selfed to obtain F2. F2 phenotypic ratio showed good fit for 3R:1S indicating resistance to powdery mildew in TARM1 line is controlled by single dominant gene. In the present study, marker MB-SSR238 (found to be linked to qPMR2) showed significant association with powdery mildew resistance through single marker analysis, explaining the phenotypic variance of 11.64 %.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810181237
dc.keywordsGeneration of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbeanen_US
dc.language.isoEnglishen_US
dc.pages96en_US
dc.publisherUniversity of Agricultural Science, Dharwaden_US
dc.research.problemGeneration of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbeanen_US
dc.subAgricultural Biotechnologyen_US
dc.themeGeneration of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbeanen_US
dc.these.typeM.Scen_US
dc.titleGeneration of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbeanen_US
dc.typeThesisen_US
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