Efficacy of phenolics from agri-byproducts in vegetable oil
| dc.contributor.advisor | Sushila Singh | |
| dc.contributor.author | Nehra, Sukriti | |
| dc.date.accessioned | 2018-11-13T09:35:48Z | |
| dc.date.available | 2018-11-13T09:35:48Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | The present study was undertaken to determine the efficacy of phenolics from pod coat of pulses in stabilising soybean oil. Pod coat of five pulse crops (cowpea, clusterbean, mungbean, Mothbean and pigeonpea) were extracted with three solvents (acetone, ethylacetate and chloroform). Phytochemical constituents (phenols, flavonoids, tocopherols and tannins) and DPPH free radical scavenging activity were estimated by using spectroscopic methods. Folin-Ciocalteu procedure and aluminium chloride colorimetric methods were used for evaluation of total phenolic content and flavonoid content respectively. Among the various extracts, highest yield was observed in acetone extract of clusterbean (5.44%) while lowest was observed in chloroform extract of cowpea (2.99%). Total phenolic content (mg GAE/g) varied from 7.24-24.18 in all of the extracts. Similarly, flavonoids (mg CAE/g) ranged from 3.30 to 10.77 in all of the extracts. Tocopherol content (mg/g) was highest in ethylacetate extract of pigeonpea (9.56) while lowest in chloroform extract of cowpea (3.41). Tannin content (mg TAE/g) was highest in acetone extract of clusterbean (2.48) while lowest in chloroform extract of mungbean (0.71). Results obtained by DPPH method showed that free radical scavenging capacities of various pod coat extracts from different species of legumes ranged from 54.95 to 78.21%. Their IC50 values (mg/mL) varied from 0.23- 0.83. Analogous to results obtained for total phenolics and flavonoids, free radical scavenging activities were highest in acetone extract of clusterbean. The high content of total phenolics and total flavonoids indicated that these bioactive metabolites contribute to synergistic effect for the antioxidant activity. Moreover, a highly significant correlation (p<0.05) between free radical scavenging efficiencies and phenolic contents as well as flavonoids was observed in most of the extracts under study. All extracts were added to crude soybean oil at two concentrations (1000 and 2000ppm). Soybean oil sample stabilized with various extracts were incubated at 50ÂșC for a period of 28 days. Effect of incorporation of these extracts on oxidative stability of crude soybean oil was measured in terms of various oxidative quality indices. Among the three extracts, acetone extract was found most effective in retarding oxidative degradation of soybean oil during incubation. Extracts were more effective at 2000ppm concentration in comparison to 1000ppm. Among the five crops, cluster bean extracts displayed highest inhibition towards oxidation of soybean oil. The results concluded that the pod coat extracts of these pulses may be valuable natural antioxidant sources and are potentially applicable in both medicine and food industry. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810083348 | |
| dc.keywords | Phenolics, Flavonoids, Pulses, Tocopherols, Tannins, Antioxidant activity | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Chemistry | en_US |
| dc.subject | null | en_US |
| dc.theme | Efficacy of phenolics from agri-byproducts in vegetable oil | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Efficacy of phenolics from agri-byproducts in vegetable oil | en_US |
| dc.type | Thesis | en_US |