CHARACTERIZATION OF ISOLATED PROTEIN FROM DE-OILED MUSTARD SEED AND EVALUATION OF ITS NUTRITIONAL AND FUNCTIONAL PROPERTIES
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Date
1992
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AAU, Anand
Abstract
With a view to find out suitability of protein isolate o[ mustard (Brassica Juncea) cv. Varuna for feed/food supplement, experiments on various aspects were conducted at the Biochemistry Department, B.A. College of Agriculture and Reproduct i.ve Biology Research Unit and Pathology Department of Veterinary College, Gujarat Agricultural University, Anand Campus, Anand during 1990-92. Dehulled, defatted meal was used for the extraction of meal nitrogen. Different conditions like, dilution ratio, time and type of equipment and pH of extracting solvent were studied to find out the optimum conditions for maximum extraction of protein from the meal. Isoelectric point was determined using 6 N HCl diid 10 per cent TCA as the precipitating reagent. To improve the colour of the protein isolate effect of sodium bisulfite and polyvinylpyrrolidone were studied at different concentrations. With the help of gel permeation chromatographic technique protein isolate was characterized and the molecular weight was also determined.
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AGRICULTURE, BIOCHEMISTRY, A STUDY