PATTERN OF ANTIBIOTIC RESISTANT MASTITIS IN A COW
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Date
2017-08
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS
Abstract
A 6 year old, HF cross breed cow was brought to the MVC Teaching Hospital
with the history of swelling of udder and reduced milk yield. Clinical examination
revealed reduced rumen motility, swelling of left forequarter, hot to touch,
painful udder, milk was dirty white in colour and milk pH was 8.5. Milk was
collected in a sterile culture tube for isolation, identification and bacteriological
examination was carried out through growth on blood agar, gram’s staining.
Staph. aureus isolates were identified asgram-positive cocci by Gram stain,
positive catalase test, growth on mannitol saltagar. Staphylococci was subjected
to antimicrobial susceptibility by disc diffusion method to the commonly used
antibiotics. Minimum inhibitory concentration was done by modified resazurin
assay microdilution technique. MIC (ugm/ml) was 125, 62.5, 125, 125, 65, 250,
0.22, 125, 250 and 250 for azathioprine, gentamicin, tetracycline, oxacillin,
cefatoxime, eefuroxime, enrofloxacin, penicillin G, ampicillin and amoxicillin.
The isolate was found sensitive for enrofloxacin, gentamicin and cefatoxime
and resistant to penicillin G, amoxicillin, ampicillin, azathioprine, tetracycline
and cefatoxime. Based on ABST & MIC, cow was treated with enrofloxacin
@ 5 mg/kg bwti/m once daily and 100 mg intramammary for 7 days, NSAID
meloxicam @ 0.5 mg/kg bwti/m once daily for 5 days, vitamin E @500 IU/day
p/o and selenium @ 6 mg / kg p/o for 7 days. PCR amplification of resistance
gene was identified.The cow showed clinical improvement after one week of
treatment.
Description
TNV_CCA_Y2017_3-4Aug.Pg193
Keywords
Veterinary Science - Conference Abstract