IDENTIFICATION OF QTL CONTROLLING POWDERY MILDEW RESISTANCE AND SEED YIELD AND ITS COMPONENTS IN BLACK GRAM (Vigna mungo (L.) Hepper)
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Date
2019-12-27
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Powdery mildew (PM) caused by Erysiphe polygoni DC is the greatest impact on
yield losses in blackgram. An attempt was made to identify resistant sources with high
seed yield to develop mapping population and identification of Quantitative trait loci
(QTL). A set of 50 germplasm were evaluated for their reaction to PM and seed yield at
K block, UAS, GKVK, Bengaluru during Kharif 2017 and genotypes LBG-17, IC-
281977, LBG-645 were resistant and high yielding. The magnitude of variation revealed
by PCV and GCV was high for PM and seed yield. QTL controlling PM resistance and
seed yield were mapped using 71 SSRs in 180 F2:3 progenies derived from the cross
TAU-1 (susceptible) × LBG-17 (Resistant). A total of 63 polymorphic markers were
assigned to eleven chromosomes of blackgram with threshold LOD of 3 covering
2726.02 cM with an average density of 43.27 cM. A major QTL for PM resistance was
detected on LG 9 at 129.00 cM, flanked by the markers CEDG166 and VrCSSSR3
with an interval of 6.00cM and explained phenotypic variance of 13.35% with negative
additive effect of -2.236 and dominant effect of 9.029. A major QTL for seed yield and
number of pods per cluster was detected on LG 4 at 180.00cM and 178.00cM
respectively, flanked by the markers, VrCSSSR2 and CEDG282 with an interval of
22.00cM and 41.00cM, and explained 15.71 and 14.28% of total phenotypic variation. It
is proposed to saturate the linkage map, confirm and validate the identified QTL for use
in marker assisted blackgram breeding.