PARTICLE GUN MEDIATED DNA DELIVERY AND TRANSIENT EXPRESSION OF -GLUCURONIDASE (GUS) GENE IN NIGER (Guizotia Abysinica Cass.)

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dc.contributor.advisorKULDEEP SINGH DANGI
dc.contributor.authorHEM RAJ BHANDARI
dc.date.accessioned2016-08-17T13:04:46Z
dc.date.available2016-08-17T13:04:46Z
dc.date.issued2006
dc.description.abstractIn the present investigation, in vitro studies were carried out to determine the optimum hormonal combination, to identify the best explant and best genotype for callus induction and shoot regeneration. Transformation studies were carried out to determine the frequency of transient GUS expression. Callus was induced from all the explants and on all the media tested. Roots recorded highest frequency of callus induction but, failed to regenerate shoots. Hypocotyls, cotyledons and primary leaves were not significantly different for callus induction. The medium with 2.0 mg/l TDZ and 0.2 mg/l NAA was found to be superior over all other combinations tested. Shoots were induced from all the explants, but cotyledonary explants recorded highest frequency of shoot induction followed by hypocotyl and primary leaf. The medium with 0.5 mg/l BA was best for shoot induction followed by medium with 0.5 mg/l TDZ. Genotypic differences were observed for both callus induction and shoot induction. CTP-local responded optimally for callus induction and JNC-6 for shoot induction. The medium with 0.5 mg/l TDZ and 0.2 mg/l NAA was best for callus induction while, the medium with 1.0 mg/l BA and 0.2 mg/l NAA was best for shoot induction. Based on the results of transformation experiments, hypocotyl were found to respond well in terms of transient GUS expression followed by primary leaf and cotyledons. Among the varieties tested, Deomali has shown highest transformability. Thus, the present study suggested direct regeneration of transgenic plants from hypocotyl, cotyledon and leaf explants and applicability of particle bombardment as an effective method of gene transfer.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/72731
dc.language.isoenen_US
dc.publisherACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABADen_US
dc.relation.ispartofseriesD7856;
dc.subGenetics and Plant Breeding
dc.subjectPARTICLE, GUN, MEDIATED, DNA, DELIVERY, TRANSIENT, EXPRESSION, GLUCURONIDASE, GENE, NIGERen_US
dc.these.typeM.Sc
dc.titlePARTICLE GUN MEDIATED DNA DELIVERY AND TRANSIENT EXPRESSION OF -GLUCURONIDASE (GUS) GENE IN NIGER (Guizotia Abysinica Cass.)en_US
dc.typeThesisen_US
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PARTICLE GUN MEDIATED DNA DELIVERY AND TRANSIENT EXPRESSION OF -GLUCURONIDASE (GUS) GENE IN NIGER (Guizotia Abysinica Cass.)
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