Preparation and characterization of streptavidinbiotin aptamer coated magnetic nanoparticle for colour based detection system of antibiotic residues in biological samples
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Date
2022
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Abstract
Aptamers are oligonucleotides that bind to a specific target molecule. Nucleic acid aptamers are usually
created by selecting them from a large random sequence pool. Mostly magnetic nanoparticles are inert in
nature, low toxicity and biocompatible. Functionalization of magnetic particle with an existing surface
and gives a new surface with possibly new properties. Magnetic particle prepared by chemical co
precipitation technique of iron salts in aqueous medium. The zeta value of -6.5Mv before coating with
aptamer and 35.6 Mv after coating with OTC specific aptamer. The nanoparticle size shows 155.4nm
before coating with OTC specific aptamer and 446.1nm after coating with aptamer. In case of coating,
250 μg of magnetic nanoparticle and 300 μg of streptavidin concentration shows good peroxidase
activity. Polymerase chain reaction of oxytetracycline aptamer with specific primer shows amplification
size 76 bp at 4% agarose gel in aptamer coated particle but negative in uncoated particle. Washing and
storing of aptamer coated particle does not remove the aptamer from the surface of magnetic particle
which indicates the aptamer and magnetic coupling is more stable. The DNA content of supernatant and
coated magnetic particles were analysed by Nano drop which shows the coated magnetic particle have
higher DNA content when compared with supernatant.
Description
TNV_IJCS_2022_10(2)11-16
Keywords
Veterinary Science