CHARACTERIZATION OF RICE (Oryza sativa L.) TRANSGENIC PLANTS CO-EXPRESSING STRESS RESPONSIVE GENES HSF4, p68 AND Pg47
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Date
2017-03-27
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Rice grown under aerobic condition experience moisture stress due to decreased water
availability and high Vapour pressure deficit ( VPD) which in turn affects the crop
growth and productivity. From this context it is important to improve adaptation of
rice under aerobic condition by improving water relations and cellular level
tolerance mechanisms. For the crops grown under rain fed conditions (moisture stress),
besides water mining, intrinsic cellular level tolerance (CLT) mechanismis important to
sustain the cell metabolism. Even with higher water mining abilities under drought, plants
experience decreased water status hence improving CLT mechanisms (osmotic
adjustment, scavenging activity and protein synthesis and turnover) is important. Among
CLT mechanisms, protein synthesis and turnover has greater significance. From this
context, for improving translational process and protein stability under stress condition,
transgenic approach was followed by co-expressing relevant stress responsive genes
namely RNA helicases and HSF4. Since multiple traits control drought tolerance, an
attempt has been made to co-express Pennisetum glaucum HSF4 (PgHSF4), Helicases
like Pennisetum glaucum 47 (Pg47) and p68 (peanut p68) in rice. The results of present
study revealed that, rice transgenics co-expressing PgHSF4, p68 and Pg47 genes were
advanced from T3 to T5 generations and evaluated their performance under stressful
environment. Several transgenic lines showed significantly improved stress tolerance in
diverse physiological screens (Salinity stress, accelerated ageing stress, temperature
stress and oxidative stress). In addition, transgenics also showed tolerance to semiirrigated
aerobic conditions in the background of a genotype (AC39020) with superior
water relations. Expression analysis of transgenes and other stress responsive
downstream genes were higher in all selected transgenics compared to wild type. The
best performing transgenic lines were selected and given for further evaluation to
understand the molecular mechanism of tolerance.
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