CLINICAL, HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL CHANGES IN VAGINAL HYPERPLASIA IN DOGS

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dc.contributor.advisorBibin Becha
dc.contributor.authorANILA BABU
dc.date.accessioned2022-10-29T06:39:26Z
dc.date.available2022-10-29T06:39:26Z
dc.date.issued2019-08-16
dc.descriptionThesis Submitted in partial fulfilment of the requirement for the degree of Master of Veterinary and Animal Sciences in Animal Reproduction, Gynaecology and Obstetrics)en_US
dc.description.abstractThe study was undertaken with the objectives of the objectives of clinico gynaecological examination, haematological studies, exfoliative vaginal cytology, ultrasonographic assessment of ovarian status, histopathological studies and immunohistochemical localisation of oestrogen receptor-α in normal oestrus dogs (Group I) and in dogs affected with vaginal hyperplasia (Group II). The overall occurrence of vaginal hyperplasia was found to be 1.02 per cent. Occurrence was more in Labrador retriever breed (48.94%), in dogs of one to two years age group (43.62%), nulliparous dogs (61.70%) and was observed during proestrus (35.11%) and oestrus (60.64%) stages of ovarian cycle in intact dogs. Presence of varying degrees of vaginal mass (86.17%), followed by vaginal discharge (25.53%) were major symptoms reported. Physiological parameters like rectal temperature, heart rate, respiratory rate and colour of mucous membranes of these dogs were within reference ranges. Clinical examination of prolapsed vaginal tissue of hyperplastic dogs revealed 83.33 per cent as Type II and 16.67 per cent as Type III vaginal hyperplasia with 50 per cent dry and pale prolapsed vaginal tissue. The mean anuclear cell index in group I and II dogs were 63.17±6.11 and 63.33±6.24, respectively. Mean recorded follicular diameter (mm) of left and right ovaries were 3.90±0.40 and 4.60±0.50 among Group I dogs, whereas in Group II it was found to be 4.70±0.50 and 4.20±0.20, respectively. Mean recorded follicular number of left and right ovaries are 3.67±0.49 and 3.50±0.42 among Group I dogs, whereas in Group II it was found to be 2.68±0.55 and 3.66±0.55 and they differed non-significantly between the groups. There was no ovarian abnormalities could be observed in Group I and Group II dogs during presented day of examination. The oestradiol levels in Group I and II were 74.11±10.52 and 82.95±11.56 pg/mL, respectively and they differed non-significantly. On histopathological analysis, oestrogenic response of the vaginal epithelium was found to be more evident in Group II than Group I dogs. The mean thickness of stratum germinativum, stratum granulosum and stratum corneum of Group I dogs were measured as 31.42±2.73, 13.93±0.73 and 32.86±6.32µm, respectively. The respective values for Group II dogs were obtained as 27.11±1.45, 13.69±1.03 and 29.77±4.10µm and no significant difference was observed between groups. The proportional score of ER-α in the vaginal epithelium was found to be significantly high (p<0.05) in Group II (3.00±0.25) than Group I dogs (1.66±0.21) and that in the stroma was found to be same (2.50 ± 0.22) in both groups. It was concluded that ER-α expression was more in vaginal epithelium of vaginal hyperplasia affected dogs.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189439
dc.keywordsVAGINAL HYPERPLASIA , DOGS, oestrous cycle, Tumours , Oestrogen therapy, Ovarian pathology, Dysuria, Exfoliative vaginal cytology, Ovarian ultrasonography, IHC localization of ER-α in vaginaen_US
dc.language.isoEnglishen_US
dc.pages125p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSURen_US
dc.research.problemExfoliative vaginal cytology and histopathological studies on vaginal mucosa of hyperplastic dogs , Ultrasonographic assessment of ovarian status in vaginal hyperplastic and normal dogs during oestrus , Comparison of oestrogen receptor-α expression in vaginal mucosa of vaginal hyperplastic and normal dogsen_US
dc.subAnimal Reproduction, Gynaecology and Obstetricsen_US
dc.themeCLINICAL, HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL CHANGES IN VAGINAL HYPERPLASIA IN DOGSen_US
dc.these.typeM.V.Sc.en_US
dc.titleCLINICAL, HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL CHANGES IN VAGINAL HYPERPLASIA IN DOGSen_US
dc.typeThesisen_US
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