STUDY ON EXPRESSION OF ANTIMICROBIAL PROTEINS IN BOVINE MILK

Simple item page
dc.contributor.advisorSingh, Geetanjali
dc.contributor.authorChandel, Sumit
dc.date.accessioned2018-07-02T09:45:02Z
dc.date.available2018-07-02T09:45:02Z
dc.date.issued2015-08
dc.description.abstractAMPs are host defense molecules and important part of the innate immunity in animals. This study was carried out to investigate and compare the expression of AMPs particularly, cathelicidins viz CATHL1, CATHL2, CATHL3 and CATHL4 in the milk of Jersey crossbred and desi pahari cows and also to compare the expression with somatic cell count. For this, 40 milk samples of Jersey crossbred cows and 10 milk samples of desi pahari cows were collected and evaluated for somatic cell counts, MCMT, BTB pH indicator test and pH. Based primarily on varying somatic cell counts, milk samples were further selected for studying the expression of these AMPs. Liver and udder tissue samples of Jersey crossbred cow were also collected for studying the expression of these AMPs. gDNA was isolated from liver tissue sample of Jersey crossbred cow. Pairs of CathL primers viz CathL1, CathL2, CathL3 and CathL4 were tested initially on gDNA. Total RNA was isolated from the liver and udder tissue samples of Jersey crossbred cow and the selected milk samples of Jersey crossbred and desi pahari cows. cDNA was then prepared from total RNA for studying the expression of these AMPs. Expression of CATHL genes viz CATHL1, CATHL2 and CATHL3 was found in liver tissue sample of Jersey crossbred cow but no expression of CATHL4 gene was detected in liver tissue sample of same Jersey crossbred cow. Expression of CATHL2 gene was found in udder tissue sample of Jersey crossbred cow but expression of CATHL1, CATHL3 and CATHL4 genes was not found in udder tissue sample of same Jersey crossbred cow. Expression of CATHL1, CATHL2, CATHL3 and CATHL4 genes was not found in the selected milk samples of Jersey crossbred and desi pahari cows. The non-expression of these AMPs might be due to either insufficient amount of mRNA specific to these proteins or alternative splicing.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810056376
dc.keywordsANTIMICROBIAL PROTEINSen_US
dc.language.isoenen_US
dc.pages62en_US
dc.publisherCSKHPKV, Palampuren_US
dc.subVeterinary Physiologyen_US
dc.subjectnullen_US
dc.themeANTIMICROBIAL PROTEINS IN BOVINE MILKen_US
dc.these.typeM.V.Sc.en_US
dc.titleSTUDY ON EXPRESSION OF ANTIMICROBIAL PROTEINS IN BOVINE MILKen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
SUMIT CHANDEL MVSc VETY BIOCHEMISTRY 2015.pdf
Size:
1.64 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses