IN VITRO AND IN VIVO MOISTURE STRESS TOLERANCE IN SUNFLOWER (Helianthus annuus L.)

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dc.contributor.advisorKULDEEP SINGH DANGI
dc.contributor.authorMANIKYAMMA, P
dc.date.accessioned2016-08-24T10:48:48Z
dc.date.available2016-08-24T10:48:48Z
dc.date.issued2005
dc.description.abstractAn investigation was carried out with six sunflower genotypes viz., SF 02, BRISF 23, BRISF 73, DSI 26, KBSH 1 and Morden with the main objective of screening the genotypes for moisture stress tolerance under in vitro and in vivo. Studies were also conducted to determine the effect of different hormonal combinations and concentrations on callus induction and shoot formation of the explants (embryo and shoot tip). Tissue culture studies indicated that embryos cultured on MS + 1 mg l-1 IAA + 0.5 mg l-1 BA higher callus initiation while, maximum multiple shoot induction was obtained on MS basal + 0.1 mg l-1 NAA + 1 mg l-1 BA. Multiple shooting from shoot tip was maximum on MS basal + 0.5 mg l-1 BA. Data were recorded for various characters, one month old seedlings both under in vitro and in vivo conditions. The seedling characters exhibited greater variation with the imposition of stress under in vitro (-0.3 MPa –0.6 MPa and –0.9 MPa) and in vivo (25%, 50% and 75% stress level). The shoot length, number of leaves per plant, shoot growth and root growth (in terms of fresh weight and dry weight) were observed to decreased progressively as the stress level increased under in vitro (-0.3 MPa to -0.9 MPa) and in vivo (25% stress to 75% stress). The shoot growth was found to be more affected than the root growth under water stress. The root / shoot ratio in terms of fresh weight and dry weight increased with increasing levels of stress under in vitro and in vivo, with highest root/shoot ratio at –0.9 MPa and 75% stress levels. Among morphological parameters studied, shoot fresh weight recorded maximum reduction followed by shoot length both under in vitro and in vivo. The proline and reducing sugar content reached maximum at 75% stress level (in vivo) in the leaf tissue. SPAD chlorophyll content, chlorophyll stability index percentage, leaf water potential and transpiration rate decreased with increasing stress level when compared to control. Among the six sunflower genotypes tested, BRISF 73 recorded minimum reduction for shoot length, shoot fresh weight, shoot dry eight, root fresh weight and root dry weight and BRISF 23 recorded more number of leaves per plant even at higher stress levels of PEG induced water stress. Under in vivo, BRISF 73 recorded minimum reduction for plant height, shoot fresh weight, shoot dry weight, where as number of leaves, root fresh weight and root dry weight recorded minimum reduction in the genotype BRISF 23 even at higher stress levels from control. The inter relationship among morphological characters of the seedlings revealed significant positive correlation between in vitro and in vivo studies for various characters. SDS PAGE analysis of total proteins will provide additional support to the findings of in vitro and in vivo at molecular level. In vitro and in vivo studies indicated that an in vitro approach could be useful in screening and selection of sunflower genotypes for their response to water stress prior to field trial. In vitro characters could provide clues for pot or field performance of sunflower genotypes grown under water stress conditions.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/73522
dc.language.isoenen_US
dc.publisherACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABADen_US
dc.relation.ispartofseriesD7462;
dc.subPlant Physiology
dc.subjectIN VITRO, IN VIVO, MOISTURE, STRESS, TOLERANCE, SUNFLOWERen_US
dc.these.typeM.Sc
dc.titleIN VITRO AND IN VIVO MOISTURE STRESS TOLERANCE IN SUNFLOWER (Helianthus annuus L.)en_US
dc.typeThesisen_US
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