Identification, characterization and functional analysis of auxin efflux carrier gene TaPIN1 in Wheat
| dc.contributor.author | Kanwardeep Singh | |
| dc.date.accessioned | 2017-03-27T10:07:02Z | |
| dc.date.available | 2017-03-27T10:07:02Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Polar auxin transport (PAT) through differentially located influx and efflux carriers’ controls many plant developmental processes. Present study is aimed at characterizing one such auxin efflux carrier gene (PIN1) in wheat using functional and translational genomics. In-silico analysis of the gene revealed the presence of conserved gene structure among monocots and dicots with six exons and five introns. Most divergent first two exons of the gene marked with monocot specific deletions/insertions code for the hydrophobic loop of the PIN1 protein. Intron evolution revealed the presence of conserved intron phases along with three ancient introns, out of which two introns showed intron sliding in some species. The proto-splice sites governed insertion of the first two introns in PIN1 gene emphasizes intron insertion after monocot-dicot divergence. Multiple amino acid sequence alignment ascertained the presence of conserved trans-membrane domains and motifs along with some species specific uncharacterized motifs in the central hydrophobic loop. Presence of the disordered protein region in the central loop and differences in the 3d structure of PIN1 protein highlights diversification of PIN1 gene among monocots and dicots as suggested by their phylogenetic analysis. BSMV-based Virus Induced Gene Silencing resulted in significant reduction of plant height (up to 30%) in wheat plants without effecting other plant traits like leaf elongation, tillering and anthesis etc. Copy number prediction and physical mapping of the PIN1 revealed the presence of atleast four copies on wheat chromosomes 6 and 7.These findings suggest the presence of functional orthologue of PIN1 gene in wheat, which can be further exploited as a candidate gene for alternate dwarfing system. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810006199 | |
| dc.keywords | Polar auxin transport, Virus Induced Gene Silencing (VIGS), Barley Stripe Mosaic Virus (BSMV), Phylogenetic analysis | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | wheat | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Identification, characterization and functional analysis of auxin efflux carrier gene TaPIN1 in Wheat | en_US |
| dc.type | Thesis | en_US |