Identification, characterization and functional analysis of auxin efflux carrier gene TaPIN1 in Wheat
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Date
2014
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Punjab Agricultural University, Ludhiana
Abstract
Polar auxin transport (PAT) through differentially located influx and efflux carriers’ controls
many plant developmental processes. Present study is aimed at characterizing one such auxin
efflux carrier gene (PIN1) in wheat using functional and translational genomics. In-silico
analysis of the gene revealed the presence of conserved gene structure among monocots and
dicots with six exons and five introns. Most divergent first two exons of the gene marked with
monocot specific deletions/insertions code for the hydrophobic loop of the PIN1 protein.
Intron evolution revealed the presence of conserved intron phases along with three ancient
introns, out of which two introns showed intron sliding in some species. The proto-splice sites
governed insertion of the first two introns in PIN1 gene emphasizes intron insertion after
monocot-dicot divergence. Multiple amino acid sequence alignment ascertained the presence
of conserved trans-membrane domains and motifs along with some species specific
uncharacterized motifs in the central hydrophobic loop. Presence of the disordered protein
region in the central loop and differences in the 3d structure of PIN1 protein highlights
diversification of PIN1 gene among monocots and dicots as suggested by their phylogenetic
analysis. BSMV-based Virus Induced Gene Silencing resulted in significant reduction of
plant height (up to 30%) in wheat plants without effecting other plant traits like leaf
elongation, tillering and anthesis etc. Copy number prediction and physical mapping of the
PIN1 revealed the presence of atleast four copies on wheat chromosomes 6 and 7.These
findings suggest the presence of functional orthologue of PIN1 gene in wheat, which can be
further exploited as a candidate gene for alternate dwarfing system.
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