EVALUATION OF SUPPLEMENTED SPENT MUSHROOM SUBSTRATE FOR THE CULTIVATION OF Pleurotus florida
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Date
2023-11-08
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation entitled, “Evaluation of supplemented spent mushroom substrate for the cultivation of Pleurotus florida” was undertaken to study the effect of supplemented spent mushroom substrate on yield and quality of Pleurotus florida.For disinfecting the substrate i.e., spent mushroom substrate, wheat straw and its combination steam pasteurization was best substrate disinfection method as it resulted in maximum yield as compared to hot water treatment. Amongst the different substrate combinations used, wheat straw resulted in maximum yield followed by combination of spent mushroom substrate + wheat straw + wheat bran + calcium carbonate (50:40:9.5:0.5). Physicochemical properties of substrate like pH, C and N content of substrates were more before cultivation and decreased after cultivation but electrical conductivity showed the reverse trend. Maximum yield and nutrients were obtained from wheat straw followed by combination of spent mushroom substrate + wheat straw + wheat bran + calcium carbonate (50:40:9.5:0.5) whereas phenol and ascorbic acid were maximum in combination of spent mushroom substrate + wheat straw + wheat bran + calcium carbonate (50:40:9.5:0.5). Enzymatic activity varied throughout the cultivation process, with laccase activity increasing from spawn run to fruit body maturation. Carboxymethyl cellulase activity was highest during primordial initiation and decreased during maturation, while xylanase activity showed increasing trend from pinning till harvesting stage.