SCREENING FOR DISEASES IN ASIATIC WILD DOGS (Cuon alpinus) IN SOUTH WAYANAD FOREST DIVISION, KERALA

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dc.contributor.advisorChintu Ravishankar
dc.contributor.authorKARKARIA TUSHNA SAROSH
dc.date.accessioned2020-07-23T06:42:41Z
dc.date.available2020-07-23T06:42:41Z
dc.date.issued2017
dc.description.abstractScreening for diseases in Asiatic wild dogs (Cuon alpinus) in Wayanad, Kerala was done from May to July 2017. The study area was South Wayanad forest division of Wayanad district of Kerala. Fresh faecal samples were collected after identification from different regions including forest paths, tea estates etc. The study was conducted to test for presence of CPV-2 in scat samples of Asiatic wild dogs in Wayanad district and to isolate and phylo-group E. coli from the samples. For detection of CPV-2, total DNA was extracted from the faecal samples by phenol – chloroform method. A PCR to detect a fragment of VP2 gene of CPV-2 was carried out. Out of the 41 samples collected 17 were positive for CPV2 (41.46%) when tested by PCR. Out of the positive samples, six were collected from tea estate (Aisha and Kurchiyarmala plantation) and seven were collected nearby KVASU campus. The faecal samples collected were also cultured on agar media to isolate E. coli. The bacteria were characterized based on the Grams staining and biochemical characteristics. The phylogrouping was then carried out employing the quadruplex PCR method described by Clermont et al. (2013). Seventeen isolates of E. coli were obtained from the 41 samples collected. Out of the 8 phylogroups A, B1, B2, C, D, E, F and Clade I, II, III, IV, V that could be detected by the quadruplex PCR methods, isolates of phylogroup A, B1, B2 and E could be detected. The identity of one isolate was unknown. Majority of the isolates belonged to group E (35.294%) followed by group B1 (29.411%) then A (23.529%) and one B2 (5.882%) and unknown (5.882%). There was no sample showing group C, D, F or clade I, II, III, IV, V.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149674
dc.keywordsSCREENING DISEASES ASIATIC WILD DOGS (Cuon alpinus) SOUTH WAYANAD FOREST DIVISION, KERALAen_US
dc.language.isoenen_US
dc.pages58en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANADen_US
dc.research.problemSCREENING FOR DISEASES IN ASIATIC WILD DOGS (Cuon alpinus) IN SOUTH WAYANAD FOREST DIVISION, KERALAen_US
dc.subWildlife Managementen_US
dc.subjectnullen_US
dc.themeSCREENING FOR DISEASES IN ASIATIC WILD DOGS (Cuon alpinus) IN SOUTH WAYANAD FOREST DIVISION, KERALAen_US
dc.these.typeM.Scen_US
dc.titleSCREENING FOR DISEASES IN ASIATIC WILD DOGS (Cuon alpinus) IN SOUTH WAYANAD FOREST DIVISION, KERALAen_US
dc.typeThesisen_US
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