SCREENING FOR DISEASES IN ASIATIC WILD DOGS (Cuon alpinus) IN SOUTH WAYANAD FOREST DIVISION, KERALA
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Date
2017
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
Screening for diseases in Asiatic wild dogs (Cuon alpinus) in Wayanad,
Kerala was done from May to July 2017. The study area was South Wayanad
forest division of Wayanad district of Kerala. Fresh faecal samples were collected
after identification from different regions including forest paths, tea estates etc.
The study was conducted to test for presence of CPV-2 in scat samples of Asiatic
wild dogs in Wayanad district and to isolate and phylo-group E. coli from the
samples.
For detection of CPV-2, total DNA was extracted from the faecal samples
by phenol – chloroform method. A PCR to detect a fragment of VP2 gene of
CPV-2 was carried out. Out of the 41 samples collected 17 were positive for CPV2 (41.46%) when tested by PCR. Out of the positive samples, six were collected
from tea estate (Aisha and Kurchiyarmala plantation) and seven were collected
nearby KVASU campus.
The faecal samples collected were also cultured on agar media to isolate
E. coli. The bacteria were characterized based on the Grams staining and
biochemical characteristics. The phylogrouping was then carried out employing
the quadruplex PCR method described by Clermont et al. (2013). Seventeen
isolates of E. coli were obtained from the 41 samples collected. Out of the 8
phylogroups A, B1, B2, C, D, E, F and Clade I, II, III, IV, V that could be detected
by the quadruplex PCR methods, isolates of phylogroup A, B1, B2 and E could
be detected. The identity of one isolate was unknown. Majority of the isolates
belonged to group E (35.294%) followed by group B1 (29.411%) then A
(23.529%) and one B2 (5.882%) and unknown (5.882%). There was no sample
showing group C, D, F or clade I, II, III, IV, V.
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