Varietal identification and seed vigour assessment in fodder sorghum [Sorghum bicolor (L.) Moench]

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dc.contributor.advisorVerma, S.S.
dc.contributor.authorGend Dnyaneshwar Gajanan
dc.date.accessioned2016-09-26T10:15:41Z
dc.date.available2016-09-26T10:15:41Z
dc.date.issued2014
dc.description.abstractThe present investigation comprised of 12 varieties of fodder sorghum (Sorghum bicolor (L.) Moench) was undertaken for varietal identification based on morphological and SDS-PAGE and to assess their vigour potential. The results revealed that morphological character like leaf sheath anthocyanin colouration, leaf midrib colour, flag leaf yellow colouration of midrib, lemma arista formation, anther colour of dry anther, glume colour, glume length, panicle length without peduncle, panicle shape, caryopsis colour and grain luster were found important diagnostic characters for categorizing these varieties. Seed characters such as seed size, seed shape and test weight clearly differentiated the varieties into different groups. Total soluble seed protein analysis using SDS–PAGE revealed that maximum total number of bands were 17. A wide quantitative variation (having different Rm values) was observed in pattern of protein bands, their electrophoretic mobility, position and intensity. The standard protein marker bands [2 (Rm 0.2 4), 6 (Rm 0.40), 9 (Rm 0.53), 11 (Rm 0.64), 13 (Rm 0.68), 16 (Rm 0.88) and 17 ( Rm 0.91)] were observed invariably common in almost all the varieties. All the varieties were differentiated by using the presence/absence of specific combination of bands or single band. The similarity was calculated in all the combination and the similarity index values ranged from 0.143 to 1.00 and the variety PC 3 gave less value with the comparison of other varieties showed dissimilarity with other remaining varieties. The dendrogram showed two major clusters at coefficient of 0.7774, the varieties PC- 3 and PC 7 in first cluster and rest varieties in second cluster. The maximum vigour potential was shown by HJ-513, HJ-541, SSV-84, HC- 136 and CSV-15 varieties which showed superiorly form the all other variety. The standard germination, accelerated ageing test, tetrazolium test and electrical conductivity were found the most suitable vigour parameters for prediction of seedling establishment in field conditions.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/78793
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subSeed Science and Technology
dc.subjectVarietal identification, SDS-PAGE, Vigour, Morphological characters.en_US
dc.these.typeM.Sc
dc.titleVarietal identification and seed vigour assessment in fodder sorghum [Sorghum bicolor (L.) Moench]en_US
dc.typeThesisen_US
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