Varietal identification and seed vigour assessment in fodder sorghum [Sorghum bicolor (L.) Moench]
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Date
2014
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Publisher
CCSHAU
Abstract
The present investigation comprised of 12 varieties of fodder sorghum (Sorghum
bicolor (L.) Moench) was undertaken for varietal identification based on morphological and
SDS-PAGE and to assess their vigour potential. The results revealed that morphological
character like leaf sheath anthocyanin colouration, leaf midrib colour, flag leaf yellow
colouration of midrib, lemma arista formation, anther colour of dry anther, glume colour,
glume length, panicle length without peduncle, panicle shape, caryopsis colour and grain
luster were found important diagnostic characters for categorizing these varieties. Seed
characters such as seed size, seed shape and test weight clearly differentiated the varieties
into different groups. Total soluble seed protein analysis using SDS–PAGE revealed that
maximum total number of bands were 17. A wide quantitative variation (having different
Rm values) was observed in pattern of protein bands, their electrophoretic mobility,
position and intensity. The standard protein marker bands [2 (Rm 0.2 4), 6 (Rm 0.40), 9 (Rm
0.53), 11 (Rm 0.64), 13 (Rm 0.68), 16 (Rm 0.88) and 17 ( Rm 0.91)] were observed
invariably common in almost all the varieties. All the varieties were differentiated by using
the presence/absence of specific combination of bands or single band. The similarity was
calculated in all the combination and the similarity index values ranged from 0.143 to 1.00
and the variety PC 3 gave less value with the comparison of other varieties showed
dissimilarity with other remaining varieties. The dendrogram showed two major clusters at
coefficient of 0.7774, the varieties PC- 3 and PC 7 in first cluster and rest varieties in
second cluster. The maximum vigour potential was shown by HJ-513, HJ-541, SSV-84, HC-
136 and CSV-15 varieties which showed superiorly form the all other variety. The standard
germination, accelerated ageing test, tetrazolium test and electrical conductivity were found
the most suitable vigour parameters for prediction of seedling establishment in field
conditions.
Description
Keywords
Varietal identification, SDS-PAGE, Vigour, Morphological characters.