In vitro studies on mass multiplication in stevia rebaudiana (Bertoni) Hemsl

Simple item page
dc.contributor.advisorYadav, R.C
dc.contributor.authorNarang, Jagriti
dc.date.accessioned2016-11-23T09:25:35Z
dc.date.available2016-11-23T09:25:35Z
dc.date.issued2006
dc.description.abstractThe present study was undertaken to develop an efficient micropropagation system for plant regeneration and transfer the regenerated plants to soil conditions in Stevia rebaudiance (Bertoni) Hemsl. Nodal and shoot tip explants were used for shoot regeneration on 39 different media supplemented with different growth regulators. Nodal explants gave best shoot regeneration response (58.70%) on MS medium supplemented with BAP (5.0 mg/L) and AgNO3 (2.0 mg/L) and MS medium fortified with BAP (5.0 mg/L)and AgNO3 (2.0 mg/L) gave highest percent of shoot initiation (71.37%) in shoot tip explants. The shoot were elongated on MS medium containing different concentrations of growth regulators using agar as gelling agent. Best shoot elongation was observed on MS medium containing BAP (4.0 mg/L), GA3 (1.0 mg/L) and AgNO3 (1.0 mg/L) out of the four media tried. Shoot tip explants are considered to be the best explant compared to nodal explants for shoot regeneration. Media supplemented with AGNO3 produce healthy shoots as compared to other media tried. Rooting of the regenerated shoots was observed (35.00%) on MS medium supplemented with IAA (2.0 mg/L) out of 9 different media tried. The regenerated plantlets were transferred to soil and vermiculite mixture (1:1 and 58.3 per cent) plants survived in the soil.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/87157
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiotechnology and Molecular Biology
dc.subjectStevia rebaudiana (Bertoni) Hemsl., Micropropagationen_US
dc.these.typeM.Sc
dc.titleIn vitro studies on mass multiplication in stevia rebaudiana (Bertoni) Hemslen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
CCSHAU-290955-Narang, Jagriti.pdf
Size:
1.96 MB
Format:
Adobe Portable Document Format
Description:
In vitro studies on mass multiplication in stevia rebaudiana (Bertoni) Hemsl
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
M. Sc. Dissertations