In vitro studies on mass multiplication in stevia rebaudiana (Bertoni) Hemsl
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Date
2006
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Publisher
CCSHAU
Abstract
The present study was undertaken to develop an efficient
micropropagation system for plant regeneration and transfer the regenerated
plants to soil conditions in Stevia rebaudiance (Bertoni) Hemsl. Nodal and shoot
tip explants were used for shoot regeneration on 39 different media
supplemented with different growth regulators. Nodal explants gave best shoot
regeneration response (58.70%) on MS medium supplemented with BAP (5.0
mg/L) and AgNO3 (2.0 mg/L) and MS medium fortified with BAP (5.0 mg/L)and AgNO3 (2.0 mg/L) gave highest percent of shoot initiation (71.37%) in
shoot tip explants.
The shoot were elongated on MS medium containing different
concentrations of growth regulators using agar as gelling agent. Best shoot
elongation was observed on MS medium containing BAP (4.0 mg/L), GA3 (1.0
mg/L) and AgNO3 (1.0 mg/L) out of the four media tried. Shoot tip explants are
considered to be the best explant compared to nodal explants for shoot
regeneration. Media supplemented with AGNO3 produce healthy shoots as
compared to other media tried. Rooting of the regenerated shoots was observed
(35.00%) on MS medium supplemented with IAA (2.0 mg/L) out of 9 different
media tried. The regenerated plantlets were transferred to soil and vermiculite
mixture (1:1 and 58.3 per cent) plants survived in the soil.
Description
Keywords
Stevia rebaudiana (Bertoni) Hemsl., Micropropagation