Characterization of Lentil (Lens culinaris Medik) genotypes using SDS-PAGE analysis , laboratory screening techniques and salt tolerance
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Date
2004-07
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In the present investigation, the characterization of 25 lentil genotypes including two local lines was carried using seed morphological, chemical and biochemical characteristics this was followed by their preliminary laboratory characteristics under induced salt tolerance, and studying the causations between seedling characteristics under induced salt stress conditions. The results revealed that the morphological parameters of lentil seed like seed coat colour, cotyledon colour, etc. in combination with chemical parameters like phenol test, NaOH bleach test, KOH leach test and biochemical parameters like SDS-PAGE protein profile of the total seed protein can effectively be used in order to characterize and develop identification key for any number of lentil genotypes. It was also found that viability and germination percentage were highly and positively correlated toeachother and same pattern was observed between plumule and radicle length. Plumule length was significantly and positively correlated to radicle length at all salt concentrations and based on the radicle length as a selection parameter for salt tolerant, three genotypes viz., VL 124, PL 015 and Local 2 were identified as salt tolerant genotypes.
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