Transcript Profiling of Pattern Recognition receptors in a Semi Domesticated Breed of Buffalo, Toda, of India

Simple item page
dc.contributor.authorVignesh, A.R.
dc.contributor.authorDhanasekaran, S.
dc.contributor.authorRaj, G. Dhinakar
dc.contributor.authorBalachandran, C.
dc.contributor.authorPazhanivel, N.
dc.contributor.authorSreekumar, C.
dc.contributor.authorTirumurugaan, K.G.
dc.contributor.authorRaja, A.
dc.contributor.authorKumanan, K.
dc.contributor.authorTANUVAS
dc.date.accessioned2016-07-20T16:18:50Z
dc.date.available2016-07-20T16:18:50Z
dc.date.issued2012
dc.description.abstractThe primary objective of this study was to assess the expression profile and levels of toll-like receptor (TLR) mRNAs in the spleen, lung, mediastinal lymph node (MLN), jejunum, rectum, skin and peripheral blood mononuclear cells (PBMC) of Toda and Murrah buffalos. Spleen and PBMC had increased expression of TLR mRNAs 2, 4, 5, 6, 8, 9 and 10; lung had increased expression of TLR mRNAs 2, 4, 5, 6 and 8, MLN TLR mRNA 6, 9, 10 and decrease in TLR 3 and 7 mRNAs in skin. No significant differences were observed in the expression levels of any of the TLR mRNA in jejunum and rectum. Toda buffaloes showed significantly higher expression levels of TLR 9 mRNA in MLN, TLR mRNAs 1, 5, 6, 9 and 10 in skin and TLR mRNAs 2, 4, 7 and 9 in PBMC than Murrah buffaloes living in the vicinity. Toda and Murrah buffaloes were inoculated with TLR5 (flagellin) and TLR9 (CpG ODN) ligands in vivo and expression levels of the respective TLRs analyzed 12 h later. Following CpG inoculation, Toda buffaloes had significantly higher levels of TLR 9 mRNA expression but not in Murrah. However, flagellin induction did not increase TLR 5 mRNA expression in both these breeds. Histological sections of the skin were made and infiltrating cell clusters were graded and quantified. Following CpG inoculation, Toda buffaloes showed higher numbers of infiltrating grade 1 and grade 3 cell clusters while Murrah showed lower numbers of infiltrating grade 1 cells as compared to mock-inoculated skin sections. Flagellin treatment revealed no significant differences in infiltrating cell clusters in both the breeds. The results have shown differential expression of TLR mRNAs in various tissues between two divergent buffalo breeds with the highest difference in TLR expression profile seen in the skin, the largest portal of entry of pathogens, of Toda.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/69067
dc.language.isoenen_US
dc.subjectToll like receptoren_US
dc.subjectTodaen_US
dc.subjectMurrahen_US
dc.subjectQuantitative real time PCRen_US
dc.subjectTLR ligandsen_US
dc.titleTranscript Profiling of Pattern Recognition receptors in a Semi Domesticated Breed of Buffalo, Toda, of Indiaen_US
dc.title.alternativeVII_147_2012_51-59en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
VII_147_2012_51-59.pdf
Size:
264.28 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)