DEVELOPMENT OF SEEDLESS GRAPE HYBRIDS THROUGH EMBRYO RESCUE AND CONFIRMATION OF HYBRIDITY BY MOLECULAR MARKERS

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dc.contributor.advisorSAHIJRAM, LEELA.
dc.contributor.authorBHASKARAREDDY, K. S.
dc.date.accessioned2017-03-17T05:58:25Z
dc.date.available2017-03-17T05:58:25Z
dc.date.issued2008-08-08
dc.description.abstractA study on development of seedless grape hybrids through embryo rescue and confirmation of hybridity by molecular markers was carried out with the objective of rescuing embryos in crosses involving ‘Thompson seedless’ as the female parent and four downy mildew resistant lines (SV12309, SV23501, SV12364, SV18315) as the pollen parents, to identify hybrids resistant to downy mildew (fungal) pathogen Plasmopara viticola, and, to develop molecular markers for grape hybrids so-generated. Using conventional breeding procedures, it is not possible to obtain hybrid progeny in grape crosses involving seedless (‘stenospermocarpic’) female parents. Such crosses can, however, be salvaged using sequential embryo rescue and hybrids can be obtained. For culturing ovules, berries of various crosses were collected at 8, 9, 10 and 11 Weeks Post Pollination (WPP). Initiation of growth and maintenance of ovules was done on Emershad and Ramming semi-solid medium supplemented with Casein hydrosylate (50mg/l)+ Myo-inositol (50mg/l)+ LCysteine (211.6 mg/l)+ Morpholino Ethane Sulphonic acid (1000mg/l). At 8 weeks from in vitro incubation, embryos were excised from ovules and subcultured on semi-solid Woody Plant Medium (WPM) supplemented with benzyl adenine (BA) @ 0.25mg/l. In four to eight weeks, the embryos started germinating and subsequently developed into full-fledged plantlets in liquid medium. Hybrid plantlets were then transferred to polybags containing Soilrite ( 75% Peat moss + 25% Perlite). A total of 122 hybrid plantlets were recovered from 955 embryos sub-cultured (which were excised from 1698 cultured ovules). The true hybrid nature of these hybrids was confirmed by using ISSR (UBC-807, UBC-817, UBC-825, UBC-828) markers.en_US
dc.identifier.otherTh-9111
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810005162
dc.language.isoenen_US
dc.pages134en_US
dc.publisherUniversity of Agricultural Sciences GKVK, Bangaloreen_US
dc.subPlant Biotechnologyen_US
dc.subjectnullen_US
dc.themeDEVELOPMENT OF SEEDLESS GRAPEen_US
dc.these.typeM.Scen_US
dc.titleDEVELOPMENT OF SEEDLESS GRAPE HYBRIDS THROUGH EMBRYO RESCUE AND CONFIRMATION OF HYBRIDITY BY MOLECULAR MARKERSen_US
dc.typeThesisen_US
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