Molecular characterization of canine adenovirus type 2 in dogs from India
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Date
2021-04
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Abstract
A total of 26 nasal swab samples were collected
from dogs with gastroenteritis and respiratory tract infections
in and around Chennai, India during 2019–20. All the
samples were subjected to PCR using common primers for
rapid diagnosis and differentiation of CAV1 and CAV2.
Only one sample produced an amplicon of 1030 bp indicating
the presence of CAV2 which was confirmed by
further sequencing. The analysis of the sequence revealed
100 per cent identity with other CAV type 2 isolates from
Brazilian, Canadian and USA strains and 95.9 per cent
identity with other Indian CAV2 strains. The phylogenetic
analysis of E3 gene reveal two distinct clusters (Asian and
America-Europe subgroup) in which our strain (ABT/
MVC/CAV2/001) grouped with CAV2 of America-Europe
subgroup instead of Asian continent subgroup.This study
confirms a novel CAV2 strain using molecular techniques
which are genetically distinct in nature from other Indian
CAV2 strains that is currently circulating in India.
Description
TNV_VD_2021_32(2)369-374
Keywords
Veterinary Science