PREVALENCE AND MOLECULAR CHARACTERIZATION OF CANINE PARVOVIRUS FROM DIARRHOEIC DOGS
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Date
2009
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Anand Agricultural University, Anand
Abstract
Canine parvovirus (CPV), a member of the genus Parvovirus, family
Parvoviridae is a non-enveloped, single-stranded DNA virus of approximately 5 kb
genome. The virus was first described in 1978, with the original isolate being termed
CPV type 2 (CPV-2). In 1979, a variant CPV strain designated CPV type 2a (CPV-2a)
became widespread, followed by further antigenic variants designated CPV type 2b
(CPV-2b) and 2c (CPV-2c), which emerged in 1984 and 2000, respectively. In due
course, CPV-2a, CPV-2b and 2c replaced CPV-2. The vaccines presently in use are
CPV-2 strain specific. Hence, the objective of the present study was to detect and
genotype CPV at different locations of Gujarat state by Polymerase chain reaction
(PCR), using primer sequences derived from the conserved VP2 region of the
genome, and to subsequent Restriction fragment length polymorphism (RFLP)
analysis of the PCR product. The RFLP analysis employed the REs HphI and MboII
in order to differentiate the CPV-2 antigenic variants. A few epidemiological factors
like breed, age, sex, season and vaccination status affecting the infection were also
studied. Haemagglutination (HA) and Haemagglutination inhibition (HI) tests were
also employed so as to compare their sensitivity with PCR.
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Keywords
Veterinary Microbiology, Analysis