PREVALENCE AND MOLECULAR CHARACTERIZATION OF CANINE PARVOVIRUS FROM DIARRHOEIC DOGS

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dc.contributor.advisorDr. M. K. Jhala
dc.contributor.authorUMRETIYA SANDIP DHIRAJLAL
dc.date.accessioned2017-06-27T10:56:42Z
dc.date.available2017-06-27T10:56:42Z
dc.date.issued2009
dc.description.abstractCanine parvovirus (CPV), a member of the genus Parvovirus, family Parvoviridae is a non-enveloped, single-stranded DNA virus of approximately 5 kb genome. The virus was first described in 1978, with the original isolate being termed CPV type 2 (CPV-2). In 1979, a variant CPV strain designated CPV type 2a (CPV-2a) became widespread, followed by further antigenic variants designated CPV type 2b (CPV-2b) and 2c (CPV-2c), which emerged in 1984 and 2000, respectively. In due course, CPV-2a, CPV-2b and 2c replaced CPV-2. The vaccines presently in use are CPV-2 strain specific. Hence, the objective of the present study was to detect and genotype CPV at different locations of Gujarat state by Polymerase chain reaction (PCR), using primer sequences derived from the conserved VP2 region of the genome, and to subsequent Restriction fragment length polymorphism (RFLP) analysis of the PCR product. The RFLP analysis employed the REs HphI and MboII in order to differentiate the CPV-2 antigenic variants. A few epidemiological factors like breed, age, sex, season and vaccination status affecting the infection were also studied. Haemagglutination (HA) and Haemagglutination inhibition (HI) tests were also employed so as to compare their sensitivity with PCR.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810023548
dc.keywordsMOLECULAR CHARACTERIZATION OF CANINE PARVOVIRUS FROM DIARRHOEIC DOGS”, CANINE PARVOVIRUS FROM DIARRHOEIC DOGS, DIARRHOEIC DOGS”en_US
dc.language.isoenen_US
dc.publisherAnand Agricultural University, Ananden_US
dc.research.problemPREVALENCE AND MOLECULAR CHARACTERIZATION OF CANINE PARVOVIRUS FROM DIARRHOEIC DOGSen_US
dc.subVeterinary Microbiologyen_US
dc.subjectVeterinary Microbiologyen_US
dc.subjectAnalysisen_US
dc.themeMOLECULAR CHARACTERIZATION OF CANINE PARVOVIRUS FROM DIARRHOEIC DOGSen_US
dc.these.typeM.V.Sc.en_US
dc.titlePREVALENCE AND MOLECULAR CHARACTERIZATION OF CANINE PARVOVIRUS FROM DIARRHOEIC DOGSen_US
dc.typeThesisen_US
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