Molecular characterization and inheritance of yellow rust resistance in wheat (Triticum aestivum L. em. Thell.)
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Date
2017
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CCSHAU
Abstract
The present investigation entitled “Molecular characterization and inheritance of yellow rust
resistance in wheat (Triticum aestivum L. em. Thell.)”, was consisted of six generations i.e. parents (P1
and P2), F1, F2, BC1P1 and BC1P2 of eight crosses viz., DBW 17 x WH 1105, DBW 17 x RAJ 3765,
DBW 17 x WH 1021, DBW 17 x WH 542, LASSIK x WH 542, LASSIK x WH 1021, LASSIK x RAJ
3765 and LASSIK x WH 1105 to study the inheritance and molecular aspects of yellow rust resistance
and genetics of quantitative traits including grain yield and its component traits. The experiments were
conducted in the Department of Genetics and Plant Breeding, CCS Haryana Agricultural University
(CCSHAU), Hisar, India during two successive years of Rabi seasons 2014-15 and 2015-16. The field
results have shown that inheritance of yellow rust was governed by two dominant genes which were
also confirmed by SSR marker analysis. It has been observed that there was a strong association
between field results for inheritance of yellow rust and SSR marker studied. Generation mean analysis
revealed significant differences for all traits studied. The C and D scales were also significant for most
of the characters indicating thereby the presence of non-allelic interactions. Additive component was
significant for most of the characters and even as preponderant in magnitude over the dominance
component. Additive x additive (i), additive x dominance (j) and dominant x dominant (l) type of
interaction were reported significant for most of the characters. However, the magnitude and direction
of the different gene effect estimates were changing from cross to cross. Additive component was
found significant for most of the characters, however, dominance gene effects were also found
significant for some characters. Duplicate type of interaction was recorded for the characters viz., spike
weight and number of grains per spike in the cross DBW 17 x WH 1105, number of tillers per plant
and number of grains per spike in the cross DBW 17 x RAJ 3765, number of tillers per plant, grain
yield per plant and biological yield per plant in the cross DBW 17 x WH 1021, spike weight, number
of spikelets per spike, grain yield per plant and biological yield pre plant in the cross DBW 17 x WH
542, spike weight in the cross LASSIK x WH 542, plant height and number of spikelets per spike in the
cross LASSIK x WH 1021, plant height, spike length and number of grains per spike in the cross
LASSIK x RAJ 3765 and spike length in the cross LASSIK x WH 1105. The differences between GCV
and PCV values were less which showed least influence of environment. Grain yield per plant showed
significant positive correlations with spike length, spike weight, 1000 grain weight, biological yield per
plant. Total 136 SSR primers were used in the present study. Out of the 136 primers, 44 primers
(including 12 Yr specific primers) were found to be polymorphic and 83 primers were monomorphic
and 9 primers did not show amplification. It was confirmed by NTSYS-PC UPGMA cluster tree and
dendrogram that two parents were most genetically diverse and the progeny lines were interspersed
between the two parents for yellow rust in all the crosses.
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