Studies on the effect of fungicides, biocontrol agents and organic amendments against wilt of lentil incited by Fusarium oxysporum f.sp. lentis
| dc.contributor.advisor | Tewari, A.K. | |
| dc.contributor.author | Kavya, H.G. | |
| dc.date.accessioned | 2021-09-30T06:29:29Z | |
| dc.date.available | 2021-09-30T06:29:29Z | |
| dc.date.issued | 2021-09 | |
| dc.description.abstract | Lentil wilt caused by Fusarium oxysporum f.sp. lentis is a major problem in all lentil growing areas of the world as well as in India. In India lentil wilt is one of the major causes behind reduced production and productivity of lentil in recent years. Lentil wilt resulted in yield loss of 50-78 per cent from moderately infected fields and 100 per cent loss from severely infected fields. In the present investigation, efforts were made to validate different management strategies against F. oxysporum f.sp. lentis. Fungicides, Peak Max and biocontrol agents were evaluated under in-vitro conditions as well as under greenhouse conditions. However, effects of different organic amendments were studied under greenhouse conditions. Bioagents and Peak Max were also studied to know their role in induction of resistance through polyphenol oxidase activity and phenylalanine ammonia lyase. In-vitro among fungicides Azoxystrobin 18.2% +Tebuconazole 18.3% SC and Carbendazim 50% WP each at 100 ppm completely inhibited (100%) radial growth of F. oxysporum f.sp. lentis. Under greenhouse conditions seed treatment + drenching with Azoxystrobin 18.2% +Tebuconazole 18.3% SC showed significantly maximum per cent disease reduction (69.35%) followed by Carbendazim 50% WP (61.49%) each at 0.1 per cent at 45 days after sowing over inoculated control. In dual culture studies among biocontrol agents Trichoderma isolate PBTR-1 was found significantly most effective in mycelial parasitization (99.3%) of F. oxysporum f. sp. lentis followed by Th- 14 (54.42%). Among bacterial bioagents Bacillus isolate PBBSR-1 showed highest width of inhibition zone (14.36 mm) followed by PBBSR-2 (11.29 mm). In greenhouse conditions significantly maximum per cent disease reduction (30.75%) was observed in seed treatment + soil application + drenching with Trichoderma isolate PBTR-1 followed by Th-14 (11.32%) over inoculated control at 45 days after sowing. Among the tested organic amendments Neem cake showed significantly maximum per cent disease reduction (70.16%) followed by Kunapajala-3 (30.35%) at 45 days after sowing over inoculated control under greenhouse conditions. The induction of defense related enzyme polyphenol oxidase was found significantly maximum (4.54 ΔO.D./ min/g of fresh tissue) in Trichoderma isolate PBTR-1 at 72 hrs while, phenylalanine ammonia lyase activity (1.99 O.D./ min/g of fresh tissue) maximum at 48 hrs followed by Th-14 in polyphenol oxidase activity (4.25 ΔO.D./min/g of fresh tissue) and phenylalanine ammonia lyase activity (1.96 O.D./min/g of fresh tissue) after drenching at 8 days after germination. Among various treatments soil application of Neem cake (50 g/kg of soil) was found best with maximum per cent disease reduction (70.16%) followed by Azoxystrobin 18.2% +Tebuconazole 18.3% SC (69.35%), Carbendazim 50% WP (61.49%) each at 0.1 per cent, Trichoderma isolate PBTR-1 (30.75%) at 1 per cent and Kunapajala-3 (30.35%) at 10 per cent at 45 days after sowing. These treatments could be further studies in different combination under field conditions for the eco-friendly cost-effective management of lentil wilt. The integration of Trichoderma isolate PBTR-1, Neem cake and Kunapajala-3 could be tested under field conditions for the management of lentil wilt under organic farming. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810176453 | |
| dc.keywords | fungicides, biological control agents, organic amendments, wilts, lentils, Fusarium oxysporum f.sp. lentis, fungal diseases | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 124 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Lentils | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | Fungal Diseases | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Studies on the effect of fungicides, biocontrol agents and organic amendments against wilt of lentil incited by Fusarium oxysporum f.sp. lentis | en_US |
| dc.type | Thesis | en_US |