Morphological and molecular analysis of genetic diversity in sorghum (Sorghum bicolor (L.) Moench)

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dc.contributor.advisorDahiya, G.S.
dc.contributor.authorMohammad Shafiqurrahaman
dc.date.accessioned2019-03-27T06:23:53Z
dc.date.available2019-03-27T06:23:53Z
dc.date.issued2019
dc.description.abstractThe present investigation comprised of 150 forage sorghum (Sorghum bicolor (L.) Moench) germplasm was undertaken for DUS characterization, morphological and molecular diversity analysis. The results revealed that DUS characters like seedling anthocynin coloration, leaf sheath anthocyanin colouration, leaf midrib colour, lemma arista formation, colour of dry anther, length for flower with pedicel length of flower, panicle emergence, plant height up to the base of flag leaf, flag leaf yellow colouration in midrib of flag leaf, stigma anthocyanin colouration, stigma length, flower with pedicel length of flower, anther length, dry anther, glume colour, panicle length of branches, panicle density at maturity, panicle shape, neck of panicle visible above sheath, Threshability, caryopsis colour, grain shape in dorsal view, grain shape in profile view, grain textures of endosperm, grain colour of vitreous albumen and grain luster were found important for identification of germplasm. High heritability coupled with high genetic advance was found for the traits namely, plant height (cm), stem diameter (cm), number of tillers per plant, number of leaves per plant, panicle length without peduncle, 1000 seed weight, green fodder yield per plant (g) and dry fodder yield per plant (g). High heritability coupled with high genetic advance indicates that there are additive genetic effects and simple selection may be effective in the material. The genotypes were grouped in to ten clusters, six clusters and nine clusters in year 2015-2016, 2016-2017 and pooled respectively. The inter cluster distance were higher than intra cluster distances, which indicated wide genetic diversity among the accessions of different groups than those of same cluster. SSR markers were used to screen polymorphisms among 48 genotypes. Out of these 50 primers 43 primers produced amplification and 38 of them were polymorphic. Two and three-dimensional principle component analysis (PCA) also gave similar clustering of 48 genotypes. The genotypes tended to group into two clusters. The genotypes which were lying nearer to each other in the dendrogram were more similar than those lying apart. This information thus obtained can be used for breeding of forage sorghum in order to meet ever increasing demand of green fodder for the livestock.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810099515
dc.keywordsMorphological characters, GCA, Heritability, Forage Sorghum, PCVen_US
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subGenetics and Plant Breedingen_US
dc.subjectnullen_US
dc.themeMorphological and molecular analysis of genetic diversity in sorghum (Sorghum bicolor (L.) Moench)en_US
dc.these.typePh.Den_US
dc.titleMorphological and molecular analysis of genetic diversity in sorghum (Sorghum bicolor (L.) Moench)en_US
dc.typeThesisen_US
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