MOLECULAR MAPPING OF DRY ROOT ROT RESISTANCE GENE(s) IN CHICKPEA (Cicer arietinum L.)

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dc.contributor.advisorK. P. VISWANATHA
dc.contributor.authorASHWINI KARADI
dc.date.accessioned2020-07-02T06:09:40Z
dc.date.available2020-07-02T06:09:40Z
dc.date.issued2018-04-30
dc.description.abstractDry root rot (DRR) caused by Rhizoctonia bataticola (Taub.) Butler [Synonyms:Macrophomina phaseolina (Tassi) Goid] is an emerging diseases of chickpea gaining importance in the changed scenario of climate. To identify the genomic regions conferring resistance to DRR, 182 Recombinant Inbred Lines (RILs) were developed from the cross between a susceptible line BG 212 and moderately resistant line ICCV 08305. The RILs were screened in the laboratory using “Paper Towel Method”. Thirteen RILs were found as resistant, whereas 79, 78 and 12 RILs showed moderate resistance, susceptible and highly susceptible reaction, respectively. The frequency distribution for DRR disease incidence depicted a normal distribution which showed quantitative nature. RILs were classified in two categories (resistance and susceptible), exhibited a ratio of 1R:1S which gives good fit with the expected ratio and chi-square value was nonsignificant. The RIL population was genotyped with cost-effective SNP genotyping platform, comprising of 50,590 high quality non-redundant SNPs tiled on to Affymetrix® Axiom® genotyping array. Out of the 50,590 SNPs, 13,110 were highly polymorphic and used for genetic map construction. As a result, a high density genetic map with 13,110 loci spanning 1,224.11 cM with an average inter-marker distance of 0.09 cM was developed. A consistent minor QTL was found for DRR on CaLG04 explaining phenotypic variation ranging from 5.87-7.41%. Two potentially important genomic regions were identified on CaLG05 and CaLG03 contains major loci for days to 50% flowering, plant height, plant biomass per plot and grain yield per plot. Resistant ICCRIL14-69 recorded high grain yield per plot (292.33g), plant biomass per plot (610g) and maximum harvest index (62.6%) during 2016. The identified genomic regions with linked SNP markers will be further utilized for introgressing DRR resistant trait into elite chickpea cultivars through Marker-Assisted Selection (MAS) to develop dry root rot resistant cultivars.en_US
dc.identifier.citation250en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810148498
dc.language.isoenen_US
dc.pages188en_US
dc.publisherUNIVERSITY OF AGRICULTURAL SCIENCES, RAICHURen_US
dc.research.problemMOLECULAR MAPPING OF DRY ROOT ROT RESISTANCE GENE(s) IN CHICKPEA (Cicer arietinum L.)en_US
dc.subGenetics and Plant Breedingen_US
dc.subjectnullen_US
dc.themeMOLECULAR MAPPING OF DRY ROOT ROT RESISTANCE GENE(s) IN CHICKPEA (Cicer arietinum L.)en_US
dc.these.typePh.Den_US
dc.titleMOLECULAR MAPPING OF DRY ROOT ROT RESISTANCE GENE(s) IN CHICKPEA (Cicer arietinum L.)en_US
dc.typeThesisen_US
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