MOLECULAR MAPPING OF DRY ROOT ROT RESISTANCE GENE(s) IN CHICKPEA (Cicer arietinum L.)
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Date
2018-04-30
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UNIVERSITY OF AGRICULTURAL SCIENCES, RAICHUR
Abstract
Dry root rot (DRR) caused by Rhizoctonia bataticola (Taub.) Butler
[Synonyms:Macrophomina phaseolina (Tassi) Goid] is an emerging diseases of chickpea
gaining importance in the changed scenario of climate. To identify the genomic regions
conferring resistance to DRR, 182 Recombinant Inbred Lines (RILs) were developed
from the cross between a susceptible line BG 212 and moderately resistant line ICCV
08305. The RILs were screened in the laboratory using “Paper Towel Method”. Thirteen
RILs were found as resistant, whereas 79, 78 and 12 RILs showed moderate resistance,
susceptible and highly susceptible reaction, respectively. The frequency distribution for
DRR disease incidence depicted a normal distribution which showed quantitative nature.
RILs were classified in two categories (resistance and susceptible), exhibited a ratio of
1R:1S which gives good fit with the expected ratio and chi-square value was nonsignificant.
The RIL population was genotyped with cost-effective SNP genotyping
platform, comprising of 50,590 high quality non-redundant SNPs tiled on to Affymetrix®
Axiom® genotyping array. Out of the 50,590 SNPs, 13,110 were highly polymorphic and
used for genetic map construction. As a result, a high density genetic map with 13,110
loci spanning 1,224.11 cM with an average inter-marker distance of 0.09 cM was
developed. A consistent minor QTL was found for DRR on CaLG04 explaining
phenotypic variation ranging from 5.87-7.41%. Two potentially important genomic
regions were identified on CaLG05 and CaLG03 contains major loci for days to 50%
flowering, plant height, plant biomass per plot and grain yield per plot. Resistant
ICCRIL14-69 recorded high grain yield per plot (292.33g), plant biomass per plot (610g)
and maximum harvest index (62.6%) during 2016. The identified genomic regions with
linked SNP markers will be further utilized for introgressing DRR resistant trait into elite
chickpea cultivars through Marker-Assisted Selection (MAS) to develop dry root rot
resistant cultivars.
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