Effect of UV radiation on Beauveria bassiana (Balsamo) and its characterization
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
Seven Beauveria bassiana isolates viz. three procured isolates MTCC4495, MTCC
2028 and NBAIR B15, two isolates of commercial formulation (Mycojaal and Daman) and
two native isolates B. bassiana F10 and B5 were evaluated for their tolerance to UV radiation,
for their UV protection with different oil supplements and pathogenicity against nymphs of
Lipaphis erysimi and were studied for their molecular characterization. B. bassiana
commercial isolate (Mycojaal) was most UV tolerant after 60 sec of UV exposure(305 nm)
with the colony forming units of 8.66×103conidia/ml and was at par with the native isolate
F10 (8.00×103conidia/ml). B. bassiana conidial suspension supplemented with different oils
recorded maximum CFU count (9.47×103 conidia/ml) in the soybean oil supplemented
conidial suspension after 60 sec of UV exposure followed by sunflower oil, rice bran and
mustard oil. The conidial suspensions of B. bassiana (109 conidia/ml) supplemented with oils
were evaluated for their pathogenicity along with control (oil unsupplement) against nymphs
of L. erysimi. The maximum mortality (67.18 %) was recorded in conidial suspension of B.
bassiana with soybean oil which was at par with the conidial suspension supplemented with
sunflower oil (66.14 %). Conidial suspension of B. bassiana F10 supplemented with oils was
most pathogenic and recorded maximum mortality (82.42%) and was at par with the
commercial isolate Mycojaal (82.41%) and MTCC 4495 (82.38%). However, minimum
mortality 36.61 per cent was in MTCC 2028. Bioevaluation of UV exposed (60 sec) conidial
suspension of B. bassiana supplemented with potential UV protectants soybean and
sunflower oil against L. erysimi recorded maximum mortality in UV exposed suspension in
soybean oil (63.18%) which was significantly better than sunflower oil treatment (61.67%)
and control (without oil supplement) (55.55%). Fifteen fungal isolates were molecular
characterized using 11 RAPD markers. A total of 76 polymorphic fragments were amplified.
RAPD markers data revealed the Beauveria isolates have shown large differences in genetic
similarity coefficient which ranged from 0.40 to 0.86. Highest similarity was recorded in C-1
(P Isolate) and C-2 (MTCC 6097) with similarity coefficient 0.86. Lowest similarity (40 %)
was in MTCC 2028 and MTCC 4495. A single 570 bp was amplified using ITS markers.
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