STUDIES ON ACELLULAR MATRIX OF SWIM BLADDER FOR OESOPHAGOPLASTY IN RABBITS
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Date
2011-09
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA
Abstract
ABSTRACT:
The present study was carried out to evaluate the acellular matrix of fish swim bladder for cervical oesophagoplasty in rabbits. Histological examination of fresh swim bladder revealed cellularity and collagen in variable amounts. Acellular matrix of swim bladder was prepared by triton X- 100 treatment and evidenced it by H & E staining. Microbiological evaluation of fresh swim bladders revealed non pathogenic cocobacilli and the colony forming units counted were not significant in terms of microbial load. A non significant difference was noticed in the tensile strength of fresh and acellular fish swim bladders.
Oesophageal defect measuring about 0.5-0.7 cm in length and 40-50% of its circumference on the ventral aspect was reconstructed in rabbits under triflupromazine hydrochloride premedication and thiopentone sodium anesthesia. In group I oesophagorrhaphy was done with a series of interrupted sutures to the mucosa and muscular layers combinedly using 5/0 silk; while in
groups II and III oesophagoplasty using fresh and acellular swim bladder grafts respectively was performed.
The rabbits in all the groups were inactive up to 10-14 postoperative days. Dysphagia and poor appetite were the clinical signs in all the groups except in group III. Leakage or fistula formation wasn’t observed at the operative site in any animal. Elevated temperature was noticed in the early post operative period in all the animals. There were no changes in the heart and respiratory rates. No significant changes were recorded in hemoglobin, packed cell volume in all the groups. A non significant leucocytosis was
observed up to 10th postoperative day in group I, while a significant increase
was noticed on 3rd, 7th and 10th postoperative day in the fresh and acellular swim bladder grafted groups. No significant changes were observed in serum glucose and cholesterol levels in all groups. Appreciable concentrations of IL- 1 and TGF-β2 were observed in the exudate yielded animals from each group.
Radiological findings revealed severe, moderate and mild stenosis in groups I, II and III respectively by 28th postoperative day. Macroscopically graft material could not be visualized on 28th day at both luminal and extraluminal surface of the oesophagus in group III, whereas in group II, it was visible and extraluminal granulamatous structure was observed at the grafted site. By 28th day an intractable stricture formation was observed in the oesophagorrhaphy group. Histological examination revealed initiation of epithelialization and angiogenesis by 21st day and medium sized arteries by 28th day in group III; whereas initiation of angiogenesis was only observed by 28th day in group II.
To conclude the present work, the acellular swim bladder showed superiority in terms of avoiding stenosis, initiation of epithelialization and angiogenesis when compared to fresh swim bladder. Hence, acellular fish swim bladder can be used as a reconstructive material for the repair of oesophageal defects.
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