EXPRESSION OF DIFFERENT HEAT SHOCK PROTEINS DURING HEAT STRESS IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF MURRAH BUFFALO
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Date
2022
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Abstract
Heat stress has a negative impact on buffalo’s health, milk production, reproductive performance, welfare and immune defence mechanism. Thermoregulatory responses at molecular level plays a major role to confer the thermotolerance capacity to the animal by expression of highly conserved family of proteins known as heat shock proteins which regulates the protein folding mechanism. During the heat stress condition, HSPs expression is increased in various cellular compartments to counteract the adverse effect of non-specific proteins. Previous studies demonstrated only the short-term effect of in-vitro thermal stress on heat shock protein gene expression in buffalo peripheral blood mononuclear cell (PBMC), none described the changes in heat stress related genes expression during long-term exposure.
Six adult healthy Murrah buffaloes (n=6) maintained in Cattle and Buffalo Breeding Unit, PGRIAS, Kattupakkam of same lactation and age were selected for this study. PBMCs were isolated from the whole blood of Murrah buffalo by density gradient centrifugation method and they were stimulated at 34 °C or 37 °C or 40 °C for 0 hr, 24 hrs and 48 hrs. At the end of each treatment, mRNA abundance of genes related to heat shock (HSF1, HSP10, HSP40, HSP60, HSP70 and HSP90) was quantified by qPCR.
The HSF-1 mRNA expression had upregulated at 34°C for both the periods of exposures but no significant changes was observed at 37°C. Exposure to higher temperature of 40°C upregulated the mRNA expression of heat shock factor 1 at both 24 hours and 48 hours.