Molecular characterization of apple stem grooving virus
| dc.contributor.advisor | Handa, Anil | |
| dc.contributor.author | Sharma, Neeraj | |
| dc.date.accessioned | 2017-06-03T11:09:41Z | |
| dc.date.available | 2017-06-03T11:09:41Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Surveys conducted during 2012 and 2013 in 84 apple orchards of Shimla and Kinnaur districts of Himachal Pradesh revealed viral incidence ranging between 0 to 80 per cent. Chlorotic spots coupled with necrotic lesions on apple leaves were the predominant symptoms. Regional Horticultural Research Station (RHRS), Mashobra, Shimla was selected for conducting biological and serological detection of apple stem grooving virus (ASGV) and other apple viruses. Serological detection through DAC and DAS-ELISA resulted in the detection of Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) in the form of mixed infection in all the samples marked for assays. Biological detection of one of the isolates carrying infection of ASGV, ACLSV, ASPV and ApMV on herbaceous hosts resulted in the production of symptoms on Chenopodium quinoa, Nicotiana glutinosa, N. tabacum and few other hosts. Detection on woody indicators (Virginia crab, M. platycarpa, Spy 227, Jay Darling and Russian Clone) under field conditions through double grafting, grafting cum budding and double budding of inoculators and indicator budwood resulted in production of typical viral symptoms on leaves in M. platycarpa, Spy 227, Jay Darling and Russian Clone besides swelling of the graft union in case of Spy 227 indicator. Leaf samples drawn during April and May months were found suitable for the ELISA of ASGV, ACLSV and ASPV whereas petals were the best source for detection of these viruses. Association of ASGV with viral symptoms in apple was also confirmed by RT-PCR assay. Molecular characterization and sequencing of ASGV isolates followed by phylogenetic studies revealed the homology of two identified isolates with other isolates of ASGV from other parts of India. Serological indexing resulted in the selection of 13 trees of 7 cultivars free from infection of ASGV, ACLSV, ASPV and ApMV in ELISA test of 36 symptomless trees of 12 cultivars. Serological studies of different cultivars and rootstocks revealed the viral infection status of different cultivars and rootstocks screened. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810016936 | |
| dc.language.iso | en | en_US |
| dc.publisher | YSPU | en_US |
| dc.sub | Plant Pathology | |
| dc.subject | apples, biological phenomena, diseases, elisa, viruses, fruits, planting, sampling, storage structures, budding | en_US |
| dc.these.type | Ph.D | |
| dc.title | Molecular characterization of apple stem grooving virus | en_US |
| dc.type | Thesis | en_US |
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