STUDIES ON PRODUCTION POTENTIAL OF ASTAXANTHIN BY Haematococcus pluvialis
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Date
2014
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Division of Microbiology Indian Agricultural Research Institute New Delhi – 110 012, India
Abstract
Haematococcus (Chlorophyta) is widely distributed unicellular green alga which
synthesizes and accumulates high level of astaxanthin in nature. Its natural habitats
include small, often temporary, continental and coastal rock pools, water holes and
other small natural or artificial water habitats. It has been reported world-wide, and
in India its occurrence has been reported in Palampur, a town in Himachal Pradesh
situated at 32°N latitude 76°E longitude and 1300m above sea level with annual
temperature of 19°C and annual rainfall of 250 cm and above. In this paper we report
new isolates of Haematococcus from water bodies of Palampur and evaluation of
their growth characteristics, photosynthetic pigment levels and astaxanthin content.
The strains named as CCCH1 and CCCH2 showed mean chlorophyll of 13.53 and
12.28 μg/mg dry biomass, respectively in their green stage (14th day of incubation).
The total carotenoids differed as 41.89 and 29.63 μg/mg dry biomass on 28th day of
incubation. On the other hand, astaxanthin content was 39.26 and 34.54 μg/mg dry
biomass in cyst stage. 18S rDNA gene amplification and further sequencing
authenticated the identification of new isolates as Haematococcus with 100 percent
homology with H. pluvialis.
Description
t-8961
Keywords
PRODUCTION POTENTIAL ASTAXANTHIN Haematococcus pluviali