DISCOVERY OF QTLs FOR DROUGHT TOLERANCE TRAITS USING A BIPARENTAL MAPPING POPULATION OF MULBERRY SEGREGATING FOR ROOT AND WATER USE EFFICIENCY (WUE)

dc.contributor.advisorNarayanaswamy, T. K
dc.contributor.authorMADHURI, THINNALURI
dc.date.accessioned2017-08-21T07:59:21Z
dc.date.available2017-08-21T07:59:21Z
dc.date.issued2015-10-26
dc.description.abstractSoil moisture stress is one of the main factors limiting the mulberry productivity in India. Maintaining leaf water relations by better root system and maintenance of superior metabolism for positive carbon gain can enhance drought adaptability. These traits are quantitatively inherited and therefore requires a forward molecular breeding approach to pyramid them. Towards identifying QTL for these complex traits, mapping population segregating for WUE and root traits was extensively characterized for phenotypic and molecular diversity. Significant genetic variability was noticed in various physiological and biometric traits among the progeny. Root weight varied between 23.66 and 250.50 g p-1 while Δ13C, a surrogate for WUE varied between 15.65 and 21.28‰. The traits segregated in a 1:1 pattern conforming to the pseudo test cross in out beeding perennials. The F1 progeny MPR-89, MPR-120, MPR-53, MPR-68 and MPR- 123 which segregated into low Δ13C category also had high TDM, LA and R/S ratio and appeared to be the most suitable genotypes for water limited conditions. A total of 751 SSR markers were screened which revealed 453 markers polymorphic between parents. A linkage map with 14 linkage groups (LGs) with 134 linked markers had a map length of 4263.5 cM. The distance between two linked markers varied from 5.0 to 49.8 cM with an average distance of 31.80 cM. QTL analysis was performed using composite interval mapping (CIM) using QTL CARTOGRAPHER (version 2.5). The CIM identified 23, 11 and 9 QTLs controlling various traits respectively on first, second and pooled data. Further, validation of these QTLs and saturation of these regions would help the breeder to exploit root traits, WUE and associated traits for genetic improvement of mulberry varieties and also to evolve new varieties adapted to water limited conditions.en_US
dc.identifier.otherTh-11267
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810029246
dc.language.isoenen_US
dc.pages160en_US
dc.publisherUNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURUen_US
dc.subSericultureen_US
dc.subjectnullen_US
dc.themeDISCOVERY OF QTLs FOR DROUGHT TOLERANCE TRAITS USING A BIPARENTAL MAPPING POPULATION OF MULBERRY SEGREGATING FOR ROOTen_US
dc.these.typePh.Den_US
dc.titleDISCOVERY OF QTLs FOR DROUGHT TOLERANCE TRAITS USING A BIPARENTAL MAPPING POPULATION OF MULBERRY SEGREGATING FOR ROOT AND WATER USE EFFICIENCY (WUE)en_US
dc.typeThesisen_US
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