Decolorization of synthetic dyes using Pleurotus spp.spent mushroom substrate
| dc.contributor.advisor | Kapoor, Shammi | |
| dc.contributor.author | Pawandeep Kaur | |
| dc.date.accessioned | 2018-01-17T14:10:18Z | |
| dc.date.available | 2018-01-17T14:10:18Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Five Pleurotus spp. (P. florida, P. sajor-caju, P. ostreatus, P. sapidus and P. eryngii) were evaluated for dye decolorization potential using the lignolytic enzymes from spent mushroom substrate (SMS). All the five species showed potential to decolorize three synthetic dyes viz. Amido Black, Congo Red and Remazol Brilliant Blue R (RBBR) when supplemented in solid and liquid medium. The mean decolorization of all the three dyes (@25-200 ppm) in agar medium was maximal for P.sajor-cajuand least for P. sapidusafter 10th day. In liquid culture, dye decolorization potential of Pleurotusspecies ranged from 67-78% for Amido Black, 8395% for RBBR and 67-77% for Congo Red by 8th day.Cultivation of all the Pleurotus species was carried out on wheat straw substratein polybags for the generation of spent mushroom substrate (SMS) and lignin modifying enzyme production potential in SMS were tested at different growth stages i.e. 10 days interval from the day of opening of bags.Among the lignin modifying enzymes, maximum activity was obtained for laccase enzyme followed by manganese peroxidase and Lignin peroxidase.The crude enzyme extracts from SMS of three Pleurotus species (P.eryngii, P.ostreatus and P.sajor-caju) were tested for their dye decolorization potential at five different growth stages of the crop. For Amido black, maximum decolorization(94%) was observed with crude enzyme extracts of P.ostreatus (94%), and for RBBR (82%)and Congo red (88%) with P.sajor-caju. Optimization studies showed that LME medium supplemented with sucrose as C-source, ammonium oxalate as Nsource with a pH of 6.5 and incubated at 25°C resulted in maximum decolorization of 50 ppm of Amido Black dye using P. sajor-caju SMS. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810039549 | |
| dc.keywords | Pleurotus spp., Spent Mushroom Substrate (SMS), Amido Black, Congo Red, RBBR | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 106 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Decolorization of synthetic dyes using Pleurotus spp.spent mushroom substrate | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Decolorization of synthetic dyes using Pleurotus spp.spent mushroom substrate | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Decolorization of synthetic dyes using Pleurotus spp.spent mushroom substrate | en_US |
| dc.type | Thesis | en_US |