Decolorization of synthetic dyes using Pleurotus spp.spent mushroom substrate
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
Five Pleurotus spp. (P. florida, P. sajor-caju, P. ostreatus, P. sapidus and P. eryngii) were
evaluated for dye decolorization potential using the lignolytic enzymes from spent mushroom
substrate (SMS). All the five species showed potential to decolorize three synthetic dyes viz.
Amido Black, Congo Red and Remazol Brilliant Blue R (RBBR) when supplemented in solid
and liquid medium. The mean decolorization of all the three dyes (@25-200 ppm) in agar
medium was maximal for P.sajor-cajuand least for P. sapidusafter 10th day. In liquid culture,
dye decolorization potential of Pleurotusspecies ranged from 67-78% for Amido Black, 8395%
for RBBR and 67-77% for Congo Red by 8th day.Cultivation of all the Pleurotus species
was carried out on wheat straw substratein polybags for the generation of spent mushroom
substrate (SMS) and lignin modifying enzyme production potential in SMS were tested at
different growth stages i.e. 10 days interval from the day of opening of bags.Among the lignin
modifying enzymes, maximum activity was obtained for laccase enzyme followed by
manganese peroxidase and Lignin peroxidase.The crude enzyme extracts from SMS of three
Pleurotus species (P.eryngii, P.ostreatus and P.sajor-caju) were tested for their dye
decolorization potential at five different growth stages of the crop. For Amido black,
maximum decolorization(94%) was observed with crude enzyme extracts of P.ostreatus
(94%), and for RBBR (82%)and Congo red (88%) with P.sajor-caju. Optimization studies
showed that LME medium supplemented with sucrose as C-source, ammonium oxalate as Nsource
with a pH of 6.5 and incubated at 25°C resulted in maximum decolorization of 50 ppm
of Amido Black dye using P. sajor-caju SMS.
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