Characterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkin

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dc.contributor.advisorRamandeep Kaur
dc.contributor.authorJasleen Kaur
dc.date.accessioned2022-11-11T15:23:13Z
dc.date.available2022-11-11T15:23:13Z
dc.date.issued2021
dc.description.abstractRice straw is one of the most abundant, renewable, cost-effective and non-edible agricultural feedstocks. Its burning has led to air pollution, loss of soil fertility and nutrient depletion, therefore valorization of rice straw is important. Straw was found to be chemically composed of 24% hemicellulose, 41% cellulose, 9% lignin, 20% extractives and 6% ash (silica). Lignin was extracted using dilute acid pretreatment (1% H2SO4) followed by alkaline hydrolysis using NaOH. FT-IR, 1H and 13C NMR were used for structural characterization of the lignin obtained from rice straw. The ethyl acetate extract of acid solubilized lignin was found to contain p-coumaric acid, ferulic acid and caffeic acid as the major phenolic acids which were isolated using preparative thin layer chromatography. The phenolic acids were characterized using elemental analyses, FT-IR, 1H and 13C NMR spectroscopy. Amides of p-coumaric acid, ferulic acid and caffeic acid were synthesized by their reaction with propyl and butyl amines using microwave irradiation and analysed using spectral studies. Phenolic acids and their amides were evaluated for their effect on pollen germination and tube growth in pumpkin. It was concluded that at 5 ppm concentration, germination percentage and pollen tube growth of p-coumaric acid, caffeic acid and their amides was enhanced than the control. These results could be utilised in increasing pollen tube length of Cucurbita moschata while making interspecific cross between C. moschata and C. pepo in order to transfer the hull-less and disease and virus resistant character of C. moschata to C. pepo.en_US
dc.identifier.citationJasleen Kaur (2021) Characterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkin (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810189805
dc.keywordsRice straw, lignin, phenolic acids, amides, pollen germination, pollen tubeen_US
dc.language.isoEnglishen_US
dc.pages75en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemCharacterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkinen_US
dc.subChemistryen_US
dc.themeCharacterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkinen_US
dc.these.typeM.Scen_US
dc.titleCharacterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkinen_US
dc.typeThesisen_US
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